Cat #: 42-197
Apex Bioresearch Products 42-197 Apex Hot Start Master Mix, 2X, 1.5mM MgCl2 (Final), 100 Reactions/Unit
Cat #: 42-197
Apex Bioresearch Products 42-197 Apex Hot Start Master Mix, 2X, 1.5mM MgCl2 (Final), 100 Reactions/Unit
1.5mM MgCl2 (Final)
100 Reactions/Unit
Brand: Apex Bioresearch Products- Apex Hot Start Master Mix, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, high yield and high specificity
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- BLUE version also available (42-145) with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Multiple 1.25ml aliquot packaging prevents repetitive freeze-thaw cycles, Total Volume: 2.5ml
- Mix is stable refrigerated (4ºC) for at least 6 months without risk
Type
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$76.80
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1.5mM MgCl2 (Final)
100 Reactions/Unit
Brand: Apex Bioresearch Products- Apex Hot Start Master Mix, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, high yield and high specificity
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- BLUE version also available (42-145) with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Multiple 1.25ml aliquot packaging prevents repetitive freeze-thaw cycles, Total Volume: 2.5ml
- Mix is stable refrigerated (4ºC) for at least 6 months without risk
Apex Hot Start Master Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. Apex Hot Start DNA Polymerase is a modified form of Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Apex Hot Start DNA Polymerase, ammonium buffer system, dNTPs and magnesium chloride are present in Apex Hot Start Master Mix with Apex Buffer I. Each reaction requires 25ul of the 2.0X reaction mix.
For most standard applications Master Mix based on Ammonium Buffer I is the best option. It promotes robust amplification, high yield and high specificity. In certain cases Master Mix with Buffer II is the best option.
| Product Overhang | 3'-dA |
|---|---|
| Hot-Start | Yes |
| 5' - 3' Exonuclease activity | Yes |
| Concentration | 2X |
| Loading Dye | No |
| Storage | Long term storage (3 years) at -20°C Short term storage (6 months) at 4°C |
| Expiration | Product expiration date at -20 °C is stated on the tube label. |
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Can this master mix still be used if it arrives without dry ice in the package?
Yes, when left at room temperature (21 - 25°C) (up to 77°F) it is stable at least 2 months with no loss of activity.
Since polymerase activity is inhibited at room temperature, the high temperatures achieved during PCR cycling will activate the polymerase which will reduce non-specific amplification and result in a higher degree of specificity. A hot start enzyme is also recommended for high-thoughput applications.
The number of reactions is based on using 25ul of the master mix for a final reaction volume of 50ul. If desired, the reaction size may be scaled down to a final volume of 20 or 25ul by using 10ul or 12.5ul of the master mix respectively
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