FectoPRO® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 4°C to ensure long term stability.

FectoPRO® is stable for at least one year, when stored appropriately.

You can find the protocol on the FectoPRO® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/fectopro/

FectoPRO® is the reagent of choice for biomanufacturing of proteins as it has been developed for scientists interested in obtaining best possible results with mammalian Transient Gene Expression systems. FectoPRO® ensures highest transfection efficiencies and protein yields in CHO and HEK-293 cell lines grown in variety of media. Moreover FectoPRO® shows great cell viability, reproducibility and consistency in results. FectoPRO® outperforms any other commercially available reagent and allows to optimize mammalian bioproduction processes (whatever scale used) as it requires equally less DNA and reagent. For large or very-large scale experiments, you might want to consider PEIpro® as it is also very efficient in HEK-293 cells and cost-effective.

The DNA amount per mL is the same if you use either the FectoCHO™ expression system or the ExpiCHO™ expression system in ExpiCHO™ cells. However keep in mind that, with FectoCHO™, you can obtain up to twice the protein production yield at a lower cost.