Cat #: 55-322

Polyplus-Transfection 101000004 FectoVIR®-AAV, Recombinant AAV Virus Production in Suspension Cells, 100ml/Unit

Cat #: 55-322

Polyplus-Transfection 101000004 FectoVIR®-AAV, Recombinant AAV Virus Production in Suspension Cells, 100ml/Unit


Recombinant AAV Virus Production in Suspension Cells

100ml/Unit

Brand: Polyplus-transfection

  • High performance: Superior yields for rAAV production
  • Scalability: Developed for small to large scale production
  • Flexibility: Compatible with commercial culture media
  • Trustworthy: #1 transfection supplier of Gene & Cell therapy

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List Price: $12,912.40
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Recombinant AAV Virus Production in Suspension Cells

100ml/Unit

Brand: Polyplus-transfection

  • High performance: Superior yields for rAAV production
  • Scalability: Developed for small to large scale production
  • Flexibility: Compatible with commercial culture media
  • Trustworthy: #1 transfection supplier of Gene & Cell therapy

AAVs are becoming the leading viral vector in the development of gene-based advanced therapy medicinal products (ATMPs). Their success is due to their intrinsic properties: they are primarily non-integrating as they persist within cells as episomes, and have a broad tropism with eleven serotypes that have the ability to infect specific cell types. A major challenge today is manufacturing sufficient quantities of AAV in order to treat larger groups of patients. FectoVIR®-AAV is a novel class of animal free transfection reagent specifically developed for industrial scale production of recombinant AAV (rAAV) viral vectors in suspension HEK-293 cell types. FectoVIR®-AAV transfection reagent guarantees higher rAAV viral titers, improved flexibility and scalability for industrial scale manufacturing.

FectoVIR®-AAV transfection reagent is a novel chemical-based and animal-free transfection reagent that has been engineered to address the current limits in rAAV viral vector yields and industrial scalability. FectoVIR®-AAV transfection reagent is the outcome of an extensive development process in which candidates from our proprietary internal chemical library were tested in comparison to the gold standard for virus production, our PEIpro® transfection reagent. In comparison to PEIpro® and other competitors, FectoVIR®-AAV stands out with its unmatched performance for the production of rAAV viral vectors in suspension cell culture systems. FectoVIR®-AAV reproducibly improves substantially AAV production yield with up to a remarkable 10-fold increase compared to other competitors (PEImax)

Brand Polyplus-Transfection
Reagent Volume 100ml
Sterile Yes
Molecule Delivered Plasmid DNA and co-delivery of several plasmid DNA
Applications Recombinant AAV virus production in suspension cells
Virus Types Adeno-Associated Viruses (AAV)
Cell Types Mammalian producer cell lines (HEK-293, HEK-293 derivatives) grown in suspension cultures
Number of Transfections 100 ml of FectoVIR®-AAV transfection reagent is sufficient to transfect on average 50 L of cell culture
Storage Condition 5 ± 3°C
Shipping Condition Ambient
Shelf Life 18 months from manufacturing date
Provided with Protocol, certificate of analysis, certificate of origin and non-hazardous product statement

The FectoVIR®-AAV structure is confidential and a patent has been filled. PEIpro® and FectoVIR®-AAV are different both in terms of molecular structure and formulation.

You will find starting conditions as well as a range for optimization in the dedicated protocol available in the documentation tab of the FectoVIR®-AAV webpage: https://www.polyplus-transfection.com/products/fectovir-aav/. If you need further information, please contact our scientific support using support@polyplus-transfection.com.

We have successfully used FectoVIR®-AAV on suspension HEK-293 derivatives (293T, 293F) and high density derivatives (Expi293™ cells). Beta-testers confirmed that FectoVIR®-AAV works well in several commercial and proprietary suspension HEK-293 subclones.

In house, we produce both AAV-2 and AAV-5. More serotypes have been tested by our beta-testers, with comparable yield improvements.

FectoVIR®-AAV shows a remarkable improvement in terms of viral titers in suspension system and is less efficient in adherent systems for which PEIpro® remains the transfection reagent of choice.

For lentivirus production, we recommend PEIpro® the gold standard for virus production as it is currently the best product for this application, both in adherent and suspension cell culture systems.

A residual test assay is currently under development.

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