Cat #: 42-702D
Apex Bioresearch Products 42-702D Apex 5X cDNA Synthesis Supermix, First-strand cDNA synthesis, 500 x 20ul Reactions/Unit
Cat #: 42-702D
Apex Bioresearch Products 42-702D Apex 5X cDNA Synthesis Supermix, First-strand cDNA synthesis, 500 x 20ul Reactions/Unit
First-strand cDNA synthesis
500 x 20ul Reactions/Unit
Brand: Apex Bioresearch Products- Simple, fast, and sensitive first-strand cDNA synthesis kit for RT-qPCR or RT-PCR
- Preblended 5X supermix contains all the necessary components except RNA template for reverse transcription
- Enhanced, engineered MMLV Rtase has reduced RNase H activity and contains a fidelity-enhancing subunit for superior accuracy
- Potent blend of RNase inhibitor prevents RNA degradation during reaction setup and reverse transcription
- Contains optimized blend of oligo(dT) and random primers for complete RNA coverage
- Complete cDNA synthesis and PCR the same day with 15 minute (for qPCR) or 50 minute (for PCR) cDNA synthesis protocol
- Both poly(A) mRNA and total RNA can be used for first-strand cDNA synthesis, but poly(A) mRNA may give higher yields and improved purity
Guest Price:
$1,785.45
$1,785.45
List Price: $2,745.35
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First-strand cDNA synthesis
500 x 20ul Reactions/Unit
Brand: Apex Bioresearch Products- Simple, fast, and sensitive first-strand cDNA synthesis kit for RT-qPCR or RT-PCR
- Preblended 5X supermix contains all the necessary components except RNA template for reverse transcription
- Enhanced, engineered MMLV Rtase has reduced RNase H activity and contains a fidelity-enhancing subunit for superior accuracy
- Potent blend of RNase inhibitor prevents RNA degradation during reaction setup and reverse transcription
- Contains optimized blend of oligo(dT) and random primers for complete RNA coverage
- Complete cDNA synthesis and PCR the same day with 15 minute (for qPCR) or 50 minute (for PCR) cDNA synthesis protocol
- Both poly(A) mRNA and total RNA can be used for first-strand cDNA synthesis, but poly(A) mRNA may give higher yields and improved purity
Apex 5X all-in-one cDNA synthesis supermix is a convenient, ready-to-use formulation of all the reagents necessary for first-strand cDNA synthesis with the exception of the template. This optimized, 5X concentrated reaction master mix contains proprietary Reverse Transcriptase, Ribonuclease Inhibitor, dNTPs and a finely balanced ratio of Oligo(dT)s and random primers.
Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR/qPCR or placed in long-term storage at -20°C.
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