Betaine Enhancer Solution 5M is one of the most effective additives over a wide range of different templates, including GC-rich sequences and templates known to be extremely difficult to amplify. Betaine Enhancer Solution 5M lowers the DNA melting temperature and has an enhancing effect on the polymerase. 

The performance of Betaine Enhancer Solution 5M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.