Cat #: 42-149
Apex Bioresearch Products 42-149 Apex Hot Start Taq BLUE Master Mix, 2X, 1.5mM MgCl2 (Final), Buffer II, 1000 Reactions/Unit
Cat #: 42-149
Apex Bioresearch Products 42-149 Apex Hot Start Taq BLUE Master Mix, 2X, 1.5mM MgCl2 (Final), Buffer II, 1000 Reactions/Unit
1.5mM MgCl2 (Final), Buffer II
1000 Reactions/Unit
Brand: Apex Bioresearch Products- Apex Hot Start Master Mix, Blue, 1.5mM MgCl2 (Final Conc.) with balanced potassium/ammonium buffer system
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- Same as clear version, but with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Dye front runs at 100-500 bp (0.5 -1.5 % agarose gel). PCR products can be used for sequencing after PCR Clean-up
- Multiple 1.25ml aliquot packaging prevent repetitive freeze-thaw cycles - mix is stable refrigerated (4ºC) for at least 6 months without risk
$595.25
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1.5mM MgCl2 (Final), Buffer II
1000 Reactions/Unit
Brand: Apex Bioresearch Products- Apex Hot Start Master Mix, Blue, 1.5mM MgCl2 (Final Conc.) with balanced potassium/ammonium buffer system
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- Same as clear version, but with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Dye front runs at 100-500 bp (0.5 -1.5 % agarose gel). PCR products can be used for sequencing after PCR Clean-up
- Multiple 1.25ml aliquot packaging prevent repetitive freeze-thaw cycles - mix is stable refrigerated (4ºC) for at least 6 months without risk
Apex Hot Start Master Mix BLUE is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. Apex Hot Start DNA Polymerase, balanced KCL/NH4 buffer system, dNTPs and magnesium chloride are present in Apex Hot Start Master Mix w/ Buffer II for multiplex PCR. Each reaction requires 25uL of the 2.0X reaction mix.
Apex Hot Start DNA Polymerase is a modified form of Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature.
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