Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. It has a higher amino acid concentration and 2 to 5 times greater vitamin concentration than BME (Basal Medium Eagle), permitting longer periods between feedings. EMEM, 2X contains 2 times the amount of MEM, 4 times the vitamins and amino acids of the original MEM, and Earle’s salts which allows for the culturing of an even wider variety of more nutritionally fastidious cells.