Cat #: 25-403
NEST 211242 NEST GelNest Matrix, Growth Factor Reduced, 5 ml, Phenol Red-Free, LDEV-Free, 1 Bottle/Unit
Cat #: 25-403
NEST 211242 NEST GelNest Matrix, Growth Factor Reduced, 5 ml, Phenol Red-Free, LDEV-Free, 1 Bottle/Unit
Phenol Red-Free, LDEV-Free
1 Bottle/Unit
Brand: NEST- Reduced growth factor and phenol red free
- Ideal for organoid cultures
- Ideal for iSPC differentiation studies
- Use for angiogenesis and tumor invasion studies
- Guaranteed protein concentration of 8mg/mL
- Lot tested free of LDEV, bacteria and mycoplasma
- Lot tested endotoxin levels <10EU/mL
$146.45
Phenol Red-Free, LDEV-Free
1 Bottle/Unit
Brand: NEST- Reduced growth factor and phenol red free
- Ideal for organoid cultures
- Ideal for iSPC differentiation studies
- Use for angiogenesis and tumor invasion studies
- Guaranteed protein concentration of 8mg/mL
- Lot tested free of LDEV, bacteria and mycoplasma
- Lot tested endotoxin levels <10EU/mL
GelNest is a basement membrane product identical in composition and performance to Corning® Matrigel® Matrix. GelNest Matrix extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcomas using genetically identical mouse lines and SOP's. EHS tumors contain plentiful ECM proteins such as laminin (60% ), collagen IV (30%), heparan sulfate proteoglycans, nidogen (8%), and several growth factors. Part number 211242 has the same ECM composition as standard GelNest Matrix but the concentration of growth factors has been reduced and the phenol red indicator removed. This the "cleanest" version of GelNest Matrix suitable for the most sensitive assays. Equivalent to Corning: 356231, 354263.With less growth factor present cell growth and differentiation is reduced. This is ideal for culture applications that require highly specific media composition. It is also useful for gene expression studies where growth factors can be confounding variable. Likewise, phenol red can exhibit estrogenic activity in some cell lines and as the name implies, is red. Removing the phenol makes 211242 ideal for applications involving steroid sensitive cell lines and colorimetric assays. The column labeled "Typical GF Concentration in" shows the typical concentrations in growth factor reduced GelNest Matrix.Please note this formulation contains phenol red as an indicator. When frozen the color may differ from yellow to dark red. This is normal and results from the interaction of carbon dioxide, the bicarbonate buffer and phenol red. The color variation is harmless and goes away when the product reaches temperature and carbon dioxide equilibrium in the incubator. Mouse colonies regularly tested for pathogens using mouse antibody production (MAP) protocol. Murine Engelbreth-Holm-Swarm (EHS) tumors used guaranteed free of LDEV. Per lot PCR testing for a wide variety of pathogens including LDEV. Tested and lot certified free of bacteria, fungi, and mycoplasma. Lowry method used to determine protein concentrations of >8mg/mL per lot, Limulus Amoebocyte Lysate assay used to quantify endotoxins, guaranteed <4EU/mL. Gel tested for 14 days at 37°C to guarantee stability. Lot tested using for biological activity using neurite growth and organoid formation assays.
211242 has the same ECM composition as standard GelNest Matrix but the concentration of growth factors has been reduced and the phenol red indicator removed. This the "cleanest" version of GelNest Matrix suitable for the most sensitive assays.
Protein Concentration | 8-12mg |
---|---|
Storage Temp | -80°C |
Liquid Working Temperature | 4°C |
Gels At | 37°C |
Shelf Life | 5 years |
Volume | 5mL |
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