Cat #: 17-702
PCR Biosystems PB30.31-02 UltraScript 2.0 cDNA Synthesis Kit, 1 x 25ul UltrasScript 2.0 RTase & 1 x 100ul cDNA Synthesis Kit, 25 Reactions/Unit
Cat #: 17-702
PCR Biosystems PB30.31-02 UltraScript 2.0 cDNA Synthesis Kit, 1 x 25ul UltrasScript 2.0 RTase & 1 x 100ul cDNA Synthesis Kit, 25 Reactions/Unit
1 x 25ul UltrasScript 2.0 RTase & 1 x 100ul cDNA Synthesis Kit
25 Reactions/Unit
Brand: PCR Biosystems- Highly thermostable reverse transcriptase 55°C to 65°C and above
- Superior cDNA yields from 20pg to 3.5µg RNA
- Accurate reverse transcription of GC-rich and highly structured transcripts
- Advanced buffer chemistry including Mg and dNTPs
- Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos
Quantity
Type
$213.85
1 x 25ul UltrasScript 2.0 RTase & 1 x 100ul cDNA Synthesis Kit
25 Reactions/Unit
Brand: PCR Biosystems- Highly thermostable reverse transcriptase 55°C to 65°C and above
- Superior cDNA yields from 20pg to 3.5µg RNA
- Accurate reverse transcription of GC-rich and highly structured transcripts
- Advanced buffer chemistry including Mg and dNTPs
- Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos
UltraScript 2.0 Reverse Transcriptase is a robust and highly thermostable modified MMLV reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation from a wide range of RNA sample types, including GC-rich and low abundance templates.
The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers, optimised to generate high yield, non-biased cDNA from as little as 4pg RNA. As oligos are not included, UltraScript Reverse Transcriptase provides the convenience and flexibility for users to define their own priming strategy depending on the type of analysis needed. UltraScript Reverse Transcriptase gives exceptional performance with gene-specific primers, oligo-dT and random hexamers to produce high quality cDNA ideal for a variety of downstream applications. High quality cDNA synthesis for downstream qPCR analysis is essential for successful expression studies. Many factors affect cDNA synthesis including the reverse transcripase, buffer systems, enhancers and priming strategy. The PCRBIO cDNA synthesis mix removes the need for user optimisation of these critical factors.
| Packaging | 1 x 25ul UltrasScript 2.0 RTase & 1 x 100ul cDNA Synthesis Kit |
|---|---|
| Quantity | 25 Reactions/Unit |
| Reverse Transcriptase | UltraScript 2.0 Reverse Transcriptase |
| Compare To | Promega GoScript™ Reverse Transcriptase Kit, Applied Biosystems™ HighCapacity RNA-to-cDNA™ Kit, Invitrogen™ SuperScript™ IV VILO™ Master Mix, Qiagen Sensiscript RT Kit |
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Can long targets (15-20 kb) be reverse transcribed by UltraScript 2.0 RTase?
20 kb RNA reverse transcription can be achieved with all our RTases because RTases jump on and off their primed substrates. Secondary structures, unspecific binding and mis-priming can be the most common causes for the reaction ending prematurely. We advise using specific primers or oligo(dT) and optimising each aspect of the reaction, for example, the different enzyme concentrations, primers, primer concentration, temperature and temperature cycling and time. Also, the subsequent PCR/qPCR step might need optimisation. We recommend UltraScript 2.0 RTase for reverse transcribing long templates because it can sustain higher temperatures for longer, which may be necessary as the probability of secondary structures in RNA increases with its length.
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