Cat #: 17-701
PCR Biosystems PB30.12-01 UltraScript Reverse Transcriptase, 2 x 25ul UltraScript RTase & 1 x 200ul Buffer, 10,000U/Unit
Cat #: 17-701
PCR Biosystems PB30.12-01 UltraScript Reverse Transcriptase, 2 x 25ul UltraScript RTase & 1 x 200ul Buffer, 10,000U/Unit
2 x 25ul UltraScript RTase & 1 x 200ul Buffer
10,000U/Unit
Brand: PCR Biosystems- Thermostable reverse transcriptase 45°C to 55°C
- High cDNA yields from as little as 4pg total RNA
- Sensitive detection of low copy number transcripts
- Advanced buffer chemistry including Mg and dNTPs
- Sensitive and accurate reverse transcription of viral RNA
- Accurate reverse transcription of GC-rich templates
Type
$300.90
2 x 25ul UltraScript RTase & 1 x 200ul Buffer
10,000U/Unit
Brand: PCR Biosystems- Thermostable reverse transcriptase 45°C to 55°C
- High cDNA yields from as little as 4pg total RNA
- Sensitive detection of low copy number transcripts
- Advanced buffer chemistry including Mg and dNTPs
- Sensitive and accurate reverse transcription of viral RNA
- Accurate reverse transcription of GC-rich templates
UltraScript Reverse Transcriptase is a robust and thermostable reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation. The latest developments in reverse transcriptase technology and buffer chemistry allow for efficient, non-biased and sensitive cDNA synthesis.
The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers, optimised to generate high yield, non-biased cDNA from as little as 4pg RNA. As oligos are not included, UltraScript Reverse Transcriptase provides the convenience and flexibility for users to define their own priming strategy depending on the type of analysis needed. UltraScript Reverse Transcriptase gives exceptional performance with gene-specific primers, oligo-dT and random hexamers to produce high quality cDNA ideal for a variety of downstream applications. High quality cDNA synthesis for downstream qPCR analysis is essential for successful expression studies. Many factors affect cDNA synthesis including the reverse transcripase, buffer systems, enhancers and priming strategy. The PCRBIO cDNA synthesis mix removes the need for user optimisation of these critical factors.
| Packaging | 2 x 25ul UltraScript RTase & 1 x 200ul Buffer |
|---|---|
| Quantity | 10,000U/Unit |
| Compare To | Thermo Scientific™ Maxima H Minus Reverse Transcriptase, NEB ProtoScript® II Reverse Transcriptase, Promega M-MLV Reverse Transcriptase |
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Can long targets (15-20 kb) be reverse transcribed by UltraScript RTase?
20 kb RNA reverse transcription can be achieved with all our RTases because RTases jump on and off their primed substrates. Secondary structures, unspecific binding and mis-priming can be the most common causes for the reaction ending prematurely. We advise using specific primers or oligo(dT) and optimising each aspect of the reaction, for example, the different enzyme concentrations, primers, primer concentration, temperature and temperature cycling and time. Also, the subsequent PCR/qPCR step might need optimisation. We recommend UltraScript 2.0 RTase for reverse transcribing long templates because it can sustain higher temperatures for longer, which may be necessary as the probability of secondary structures in RNA increases with its length.
UltraScript RTase can transcribe as little as 4 pg and up to 0.4 ?g of RNA. The performance depends on the type of RNA, RNA quality and whether specific primers, oligod(Ts) or random hexamers are used. For higher capacity applications please use UltraScript 2.0 Reverse Transcriptase and cDNA Synthesis Kits.
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