Cat #: 17-601C
PCR Biosystems PB25.12-12 qPCRBIO SyGreen 1-Step Detect Hi-ROX, 12 x 1ml Mix & 12 x 200ul RTase, 1200 x 20ul Rxns/Unit
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Cat #: 17-601C
PCR Biosystems PB25.12-12 qPCRBIO SyGreen 1-Step Detect Hi-ROX, 12 x 1ml Mix & 12 x 200ul RTase, 1200 x 20ul Rxns/Unit
12 x 1ml Mix & 12 x 200ul RTase
1200 x 20ul Rxns/Unit
Brand: PCR Biosystems- Designed for sensitivity and is ideally suited to the detection of extremely low copy number targets
- Recommended for template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction
- Thermostable reverse transcriptase 45°C to 55°C
- Contains advanced RNase inhibitor
- Non-PCR inhibiting intercalating dye
- Rapid extension rate for early Ct values
- Market leading sensitivity - increased limit of detection
- Compatible on all real-time PCR platforms - standard and fast cycling conditions
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$1,336.00
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12 x 1ml Mix & 12 x 200ul RTase
1200 x 20ul Rxns/Unit
Brand: PCR Biosystems- Designed for sensitivity and is ideally suited to the detection of extremely low copy number targets
- Recommended for template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction
- Thermostable reverse transcriptase 45°C to 55°C
- Contains advanced RNase inhibitor
- Non-PCR inhibiting intercalating dye
- Rapid extension rate for early Ct values
- Market leading sensitivity - increased limit of detection
- Compatible on all real-time PCR platforms - standard and fast cycling conditions
qPCRBIO 1-Step SyGreen mix uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by PCRBIO Taq HS DNA Polymerase, resulting in robust RT-PCR performance. The 1-step kits can be used to quantify any RNA template inclusing mRNA, total RNA and viral RNA sequences. The 1-step detect kit is designed for high sensitivity and is suited for low copy number targets.
qPCRBIO 1-Step SyGreen mix can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation. any modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.
Reactions | 1200 x 20ul Rxns/Unit |
---|---|
Packaging | 12 x 1ml Mix & 12 x 200ul RTase |
Storage | Between -30 and -15°C |
Recommended Template Amount | 1pg - 10ng total RNA or >0.01pg mRNA per reaction |
Compare To | BIORAD iTaq Universal SYBR® Green One-Step Kit, Qiagen QuantiTect SYBR® Green RT-PCR Kit, Thermo Scientific Verso 1-step, RT-qPCR Kit, SYBR Green, ROX |
No, this kit will not work for 2-step reactions. For 2-step reactions, we recommend the qPCRBIO cDNA Synthesis Kit or UltraScript 2.0 cDNA Synthesis Kit for more difficult targets, along with real time PCR kits depending on your needs.
No, the RTase Go contains an RNase inhibitor to prevent any degradation and increase sensitivity.
qPCRBIO SyGreen 1-step Detect has been engineered to have high sensitivity for rapid and accurate results from low template concentrations 1pg of total RNA or 0.01pg of mRNA. qPCRBIO SyGreen 1-step Go has been developed for rapid (early Ct) and accurate results from high template concentrations from 10pg total RNA or 0.01pg of mRNA.
Yes, qPCRBIO SyGreen 1-Step Detect | Go can be used for micro RNA templates. Although we do no sell dedicated kits, all of our RTases can be used for miRNA quantification and analysis. Please reach out if you'd like recommended protocols.
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