Cat #: 17-207
PCR Biosystems PB10.24-08 PCRBIO Rapid Extract PCR Kit, Extract PCR-ready DNA, Includes Taq, 80 Reactions/Unit
Cat #: 17-207
PCR Biosystems PB10.24-08 PCRBIO Rapid Extract PCR Kit, Extract PCR-ready DNA, Includes Taq, 80 Reactions/Unit
Extract PCR-ready DNA, Includes Taq
80 Reactions/Unit
Brand: PCR Biosystems- Rapid, convenient, single-tube DNA extraction
- Produces high yield, PCR ready DNA in 15 minutes
- Maximized PCR speed, yield and specificity
- Powered by PCRBIO Hot Start Taq Mix Red for direct gel loading
- Suitable for multiple sample types including mouse tail clip and ear punch, animal tissue, hair follicle, buccal swab, mammalian blood and FFPE tissue
- Also available as a lysis kit without the Hot Start Taq Polymerase (Cat #:17-301)
Size
$150.80
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Extract PCR-ready DNA, Includes Taq
80 Reactions/Unit
Brand: PCR Biosystems- Rapid, convenient, single-tube DNA extraction
- Produces high yield, PCR ready DNA in 15 minutes
- Maximized PCR speed, yield and specificity
- Powered by PCRBIO Hot Start Taq Mix Red for direct gel loading
- Suitable for multiple sample types including mouse tail clip and ear punch, animal tissue, hair follicle, buccal swab, mammalian blood and FFPE tissue
- Also available as a lysis kit without the Hot Start Taq Polymerase (Cat #:17-301)
PCRBIO Rapid Extract PCR Kit from Genesee Scientific combines rapid DNA extraction with fast, highly specific DNA amplification in a convenient, easy to use format. Eliminate the need for laborious and time-consuming DNA extraction methods with this simple, integrated extraction and amplification PCR kit powered by the latest advances in hot start polymerase technology.
PCRBIO Rapid Extract PCR Kit has been developed for fast, efficient amplification of DNA from a variety of tissues and is particularly suited to solid tissue such as mouse tail or mouse ear. Sample processing is simplified and contamination risks minimized as DNA extraction is performed in a single tube, removing the need for multiple washing steps. Extracted DNA is amplified in a proprietary buffer system using PCRBIO Hot Start Taq Mix Red. Our antibody hot start polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and sensitivity. Primer-dimer formation and non-specific amplification are prevented giving superior specificity ideal for complex templates such as mammalian genomic DNA. PCRBIO Hot Start Taq Mix Red contains a red tracking dye for direct loading on agarose gels.
| Packaging | 1 x 1.6ml Buffer A, 1 x 800ul Buffer B & 2 x 1ml HS Taq Mix Red |
|---|---|
| Reactions | 80 x 50ul Rxns/Unit |
| Storage | Between -30 and -15°C |
| Compare To | Sigma Extract-N-Amp™ Tissue PCR Kit, KAPA Express Extract Kit |
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Can I use PCRBIO Rapid Extract PCR Kit to extract and amplify DNA from a few dozen cells on filter paper?
Our PCR mixes work reliably with 10 or more copies of template per reaction but not lower than that. Keep the extracted DNA in the lowest volume possible and don’t dilute further after inactivating the protease. We advise setting up the assay with more cells and once the conditions are established, scaling it down from there.
Yes, it can be used to amplify DNA from bacterial colonies or bacterial cell lysate however, it’s not necessary to use the Rapid Extract PCR Kit in order to perform routine colony PCR. If you’re working from bacterial colonies use a sterile tip to pick a colony and resuspend into the 50µl PCR reaction. If working from liquid culture add 5µl of overnight culture to the final mix. Follow the general protocol and increase the initial denaturation time to 10 min at 95°C. For qPCR, it may be important to extract DNA with the PCRBIO Rapid Extract Lysis Kit to decrease background noise and control the amount of DNA added to the reaction.
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