Cat #: 17-202
PCR Biosystems PB10.13-10 PCRBIO Taq Mix, Red, 5 x 1ml Taq Mix Red, 200 Reactions/Unit
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Cat #: 17-202
PCR Biosystems PB10.13-10 PCRBIO Taq Mix, Red, 5 x 1ml Taq Mix Red, 200 Reactions/Unit
5 x 1ml Taq Mix Red
200 Reactions/Unit
Brand: PCR Biosystems- For a variety of applications including routine PCR, TA cloning, methylated DNA, high throughput PCR, fast PCR, and many more
- Advanced buffer chemistry that includes Mg and dNTPs
- High yields under standard and fast PCR conditions
- Performs consistently well on a broad range of templates including GC rich and AT rich sequences
- Increased PCR success rates with amplicons up to 6kb
- Contains red tracking dye for direct gel loading
- Also available without dye and as a polymerase with separate reaction buffer
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5 x 1ml Taq Mix Red
200 Reactions/Unit
Brand: PCR Biosystems- For a variety of applications including routine PCR, TA cloning, methylated DNA, high throughput PCR, fast PCR, and many more
- Advanced buffer chemistry that includes Mg and dNTPs
- High yields under standard and fast PCR conditions
- Performs consistently well on a broad range of templates including GC rich and AT rich sequences
- Increased PCR success rates with amplicons up to 6kb
- Contains red tracking dye for direct gel loading
- Also available without dye and as a polymerase with separate reaction buffer
PCRBIO Taq DNA Polymerase from Genesee Scientific provides researchers with an affordable, routine application polymerase that performs to the highest possible standard and with a versatility that allows amplification at the highest speeds, yields, specificity and consistency. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA and performs consistently well on a broad range of templates including both GC and AT rich.
PCRBIO Taq is a robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. PCRBIO Taq DNA Polymerase has 5-3 exonuclease activities, but no 3-5 exonuclease (proofreading) activity. The enzyme has the same error rate as wild-type taq DNA polymerase, approximately 1 error per 2.0 x 10^5 nucleotides incorporated. PCR products generated with PCRBIO Taq DNA Polymerase are A-tailed and may be cloned into TA cloning vectors. The PCRBIO Taq DNA Polymerase is also available as a separate polymerase with separate reaction buffer or as a clear master mix with no red dye.
Packaging | 5 x 1ml Taq Master Mix Red |
---|---|
Reactions | 200 x 50ul Reactions |
Error Rate | 1 error per 2.0 x 10^5 nucleatides incorporated |
Color | Red |
Storage | Between -30 and -15°C |
Compare To | Invitrogen PCR SuperMix, DreamTaq PCR Master Mix, Promega GoTaq® Master Mix |
Yes. If youre working from bacterial colonies use a sterile tip to pick a colony and re-suspend into the 50µl PCR reaction. If working from liquid culture add 5µl of overnight culture to the final mix. Follow the general protocol and increase the initial denaturation time to 10 min at 95°C.
We recommend using PCRBIO HS Taq DNA Polymerase for amplifying DNA from blood samples. If youd like to use PCRBIO Taq DNA Polymerase, use 2 µL blood sample to a 50 µL PCR reaction and follow the general protocol. Please note that blood components may inhibit the PCR reaction. Perform a serial dilution of the sample in order to find the optimal template concentration for the PCR amplification.
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