Cat #: 17-102
PCR Biosystems PB10.21-50 PCRBIO Hot Start Taq DNA Polymerase, 1 Hot Start Taq & 2 x 1ml Buffer, 250U/Unit
Cat #: 17-102
PCR Biosystems PB10.21-50 PCRBIO Hot Start Taq DNA Polymerase, 1 Hot Start Taq & 2 x 1ml Buffer, 250U/Unit
1 Hot Start Taq & 2 x 1ml Buffer
250U/Unit
Brand: PCR Biosystems- Proprietary hot start technology for unrivalled detection of low copy number templates
- Increased PCR success rates with amplicons up to 6kb
- Advanced buffer chemistry including Mg and dNTPs
- High yields under standard and fast PCR conditions
- Efficient specific amplification from complex templates including GC rich and AT rich sequences
- Ultra low background DNA
- Also available as a ready mix or ready mix with red dye for direct gel loading
Size
$141.00
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1 Hot Start Taq & 2 x 1ml Buffer
250U/Unit
Brand: PCR Biosystems- Proprietary hot start technology for unrivalled detection of low copy number templates
- Increased PCR success rates with amplicons up to 6kb
- Advanced buffer chemistry including Mg and dNTPs
- High yields under standard and fast PCR conditions
- Efficient specific amplification from complex templates including GC rich and AT rich sequences
- Ultra low background DNA
- Also available as a ready mix or ready mix with red dye for direct gel loading
PCRBIO Hot Start Taq DNA Polymerase from Genesee Scientific uses advanced antibody-mediated hot start technology for superior sensitivity. Whether you need a hot start assay for high throughput, automated reaction set up or for detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.
“hot start” is a term used to describe the inactivation of a DNA polymerase until the initial activation step at 95°C. Inactivation below 65°C prevents primer-dimer formation and non-specific amplification allowing for specific amplification from low copy number target sequences. Our proprietary small-molecule hot start technology offers improved specificity and sensitivity compared to other methods. PCRBIO Hot Start DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO Hot Start Taq DNA Polymerase can perform consistently well on a broad range of templates (including both GC and AT rich).
| Packaging | 1 x 50ul HS Taq DNA Polymerase & 2 x 1ml 5x Reaction Buffer |
|---|---|
| Quantity | 250U/Unit |
| Error Rate | 1 error per 2.0 x 10^5 nucleatides incorporated |
| Storage | Between -30 and -15°C |
| Compare To | Platinum™ Taq, DreamTaq™ Hot Start Taq, NEB EpiMark® Hot Start Taq, Promega GoTaq® Hot Start |
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Can PCRBIO Hot Start Taq DNA Polymerase be used for Multiplex PCR?
Our PCRBIO Hot Start Taq DNA Polymerase is particularly suited for multiplexing due to the presence of our hot-start technology, which blocks the activity of Taq polymerase at low temperatures. When first performing multiplex PCR, we recommend running an annealing temperature gradient from 55°C to 65°C. The annealing temperature that results in the best specificity should be used in subsequent experiments. Fast cycling conditions should not be used for multiplex PCR. We recommend a 90 second extension time to begin with and this time may be extended to increase yield. For multiplex reactions, the reactions should be set up on ice or cooling blocs from start till finish. Primers must be designed carefully to avoid overlapping sequences as much as possible while maintaining diverse amplicon lengths that can be easily analyzed with your end-detection method
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