Cat #: 17-100C
PCR Biosystems PB10.11-40 PCRBIO Taq DNA Polymerase, 8 x 100ul Taq & 32 x 1ml Buffer, 4000U/Unit
Cat #: 17-100C
PCR Biosystems PB10.11-40 PCRBIO Taq DNA Polymerase, 8 x 100ul Taq & 32 x 1ml Buffer, 4000U/Unit
8 x 100ul Taq & 32 x 1ml Buffer
4000U/Unit
Brand: PCR Biosystems- For a variety of applications including routine PCR, TA cloning, methylated DNA, high throughput PCR, fast PCR, and many more
- Advanced buffer chemistry that includes Mg and dNTPs
- High yields under standard and fast PCR conditions
- Performs consistently well on a broad range of templates including GC rich and AT rich sequences
- Increased PCR success rates with amplicons up to 6kb
- Also available with red dye for direct gel loading or as a polymerase with separate reaction buffer
Size
$808.70
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8 x 100ul Taq & 32 x 1ml Buffer
4000U/Unit
Brand: PCR Biosystems- For a variety of applications including routine PCR, TA cloning, methylated DNA, high throughput PCR, fast PCR, and many more
- Advanced buffer chemistry that includes Mg and dNTPs
- High yields under standard and fast PCR conditions
- Performs consistently well on a broad range of templates including GC rich and AT rich sequences
- Increased PCR success rates with amplicons up to 6kb
- Also available with red dye for direct gel loading or as a polymerase with separate reaction buffer
PCRBIO Taq DNA Polymerase from Genesee Scientific provides researchers with an affordable, routine application polymerase that performs to the highest possible standard and with a versatility that allows amplification at the highest speeds, yields, specificity and consistency. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA and performs consistently well on a broad range of templates including both GC and AT rich.
PCRBIO Taq is a robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. PCRBIO Taq DNA Polymerase has 5’-3’ exonuclease activities, but no 3’-5’ exonuclease (proofreading) activity. The enzyme has the same error rate as wild-type taq DNA polymerase, approximately 1 error per 2.0 x 10^5 nucleotides incorporated. PCR products generated with PCRBIO Taq DNA Polymerase are A-tailed and may be cloned into TA cloning vectors. The PCRBIO Taq DNA Polymerase is also available as a separate polymerase with separate reaction buffer or as a master mix with a red dye for easy direct-to-gel analysis.
| Packaging | 8 x 100ul Taq DNA Polymerase and 32 x 1ml Reaction Buffer |
|---|---|
| Quantity | 4000U/Unit |
| Error Rate | 1 error per 2.0 x 10^5 nucleatides incorporated |
| Storage | Between -30 and -15°C |
| Compare To | AmpliTaq™, DreamTaq™, NEB OneTaq®, Promega GoTaq® |
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Can PCRBIO Taq DNA Polymerase be used for colony PCR?
Yes. If you’re working from bacterial colonies use a sterile tip to pick a colony and re-suspend into the 50µl PCR reaction. If working from liquid culture add 5µl of overnight culture to the final mix. Follow the general protocol and increase the initial denaturation time to 10 min at 95°C.
We recommend using PCRBIO HS Taq DNA Polymerase for amplifying DNA from blood samples. If you’d like to use PCRBIO Taq DNA Polymerase, use 2 µL blood sample to a 50 µL PCR reaction and follow the general protocol. Please note that blood components may inhibit the PCR reaction. Perform a serial dilution of the sample in order to find the optimal template concentration for the PCR amplification.
The 5x PCRBIO Reaction Buffer supplied with PCRBIO Taq DNA Polymerase has been developed specifically for this enzyme and we highly recommend using them together. However, PCRBIO Taq DNA Polymerase should be compatible with any PCR buffer developed for use with wild-type Taq. If you use a customized buffer with PCRBIO Taq DNA Polymerase, keep in mind reaction parameters such as annealing temperature and concentrations of the enzyme, template, dNTPs and MgCl2, may require optimization.
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