Cat #: 13-045
Intact Genomics 1256-48 BL21(DE3) pLysS, Electrocompetent Cells, 12 x 100µl/Unit
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Cat #: 13-045
Intact Genomics 1256-48 BL21(DE3) pLysS, Electrocompetent Cells, 12 x 100µl/Unit
Electrocompetent Cells
12 x 100µl/Unit
Brand: Intact Genomics- Suitable for transformation and routine protein expression
- Has T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
- Suitable for expression of toxic genes
- Includes: ig® BL21(DE3)pLysS electroCompetent cells, pUC19 control DNA, and recovery medium
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$505.00
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Electrocompetent Cells
12 x 100µl/Unit
Brand: Intact Genomics- Suitable for transformation and routine protein expression
- Has T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
- Suitable for expression of toxic genes
- Includes: ig® BL21(DE3)pLysS electroCompetent cells, pUC19 control DNA, and recovery medium
Intact Genomics (ig®) Electrocompetent BL21(DE3)pLysS electrocompetent cells are suitable for transformation and routine protein expression. The pLysS plasmid produces T7 lysozyme that reduces base level expression of the gene of interest, subsequently allowing for tighter control of expression and is therefore suitable for expression of toxic genes. BL21(DE3)pLysS electrocompetent cells also carry the chloramphenicol resistance gene.
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ?1 x 1010 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
Competent cell type | Electrocompetent |
---|---|
Derivative of | BL21(DE3)pLysS |
Species | E. coli |
Format | Tubes |
Transformation efficiency | ?1 x 10^10 cfu/µg pUC19 DNA |
Blue/white screening | Yes |
Shipping condition | Dry ice |
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