Cat #: 13-045

Intact Genomics 1256-48 BL21(DE3) pLysS, Electrocompetent Cells, 12 x 100µl/Unit

Intact Genomics 1256-48 BL21(DE3) pLysS, Electrocompetent Cells, 12 x 100
Intact Genomics 1256-48 BL21(DE3) pLysS, Electrocompetent Cells, 12 x 100
Intact Genomics 1256-48 BL21(DE3) pLysS, Electrocompetent Cells, 12 x 100

Cat #: 13-045

Intact Genomics 1256-48 BL21(DE3) pLysS, Electrocompetent Cells, 12 x 100µl/Unit


Electrocompetent Cells

12 x 100µl/Unit

Brand: Intact Genomics

  • Suitable for transformation and routine protein expression
  • Has T7 Expression Strain
  • Deficient in both lon (1) and ompT proteases
  • Resistant to phage T1 (fhuA2)
  • B Strain
  • Suitable for expression of toxic genes
  • Includes: ig® BL21(DE3)pLysS electroCompetent cells, pUC19 control DNA, and recovery medium

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$505.00
List Price: $555.50
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Electrocompetent Cells

12 x 100µl/Unit

Brand: Intact Genomics

  • Suitable for transformation and routine protein expression
  • Has T7 Expression Strain
  • Deficient in both lon (1) and ompT proteases
  • Resistant to phage T1 (fhuA2)
  • B Strain
  • Suitable for expression of toxic genes
  • Includes: ig® BL21(DE3)pLysS electroCompetent cells, pUC19 control DNA, and recovery medium

Intact Genomics (ig®) Electrocompetent BL21(DE3)pLysS electrocompetent cells are suitable for transformation and routine protein expression. The pLysS plasmid produces T7 lysozyme that reduces base level expression of the gene of interest, subsequently allowing for tighter control of expression and is therefore suitable for expression of toxic genes.  BL21(DE3)pLysS electrocompetent cells also carry the chloramphenicol resistance gene.

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ?1 x 1010 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

Competent cell type Electrocompetent
Derivative of BL21(DE3)pLysS
Species E. coli
Format Tubes
Transformation efficiency ?1 x 10^10 cfu/µg pUC19 DNA
Blue/white screening Yes
Shipping condition Dry ice

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