Cat #: 13-042

Intact Genomics 1252-24 BL21(DE3), Electrocompetent Cells, 12 x 50µl/Unit

Intact Genomics 1252-24 BL21(DE3), Electrocompetent Cells, 12 x 50
Intact Genomics 1252-24 BL21(DE3), Electrocompetent Cells, 12 x 50
Intact Genomics 1252-24 BL21(DE3), Electrocompetent Cells, 12 x 50

Cat #: 13-042

Intact Genomics 1252-24 BL21(DE3), Electrocompetent Cells, 12 x 50µl/Unit

Electrocompetent Cells

12 x 50µl/Unit

Brand: Intact Genomics
  • Suitable for high efficiency transformation and routine protein expression
  • Contains >1 x 1010 cfu/µg efficiency with electroporation
  • Widely used host background
  • Has T7 Expression Strain
  • Deficient in both lon (1) and ompT proteases
  • Resistant to phage T1 (fhuA2)
  • B Strain
  • Includes: BL21(DE3) electrocompetent cells, pUC19 control DNA, and recovery medium
Guest Price:
$225.00
List Price: $247.50
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Electrocompetent Cells

12 x 50µl/Unit

Brand: Intact Genomics
  • Suitable for high efficiency transformation and routine protein expression
  • Contains >1 x 1010 cfu/µg efficiency with electroporation
  • Widely used host background
  • Has T7 Expression Strain
  • Deficient in both lon (1) and ompT proteases
  • Resistant to phage T1 (fhuA2)
  • B Strain
  • Includes: BL21(DE3) electrocompetent cells, pUC19 control DNA, and recovery medium

Intact Genomics BL21 (DE3) electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21(DE3) electrocompetent E. coli cells ideal for construction of complex expression libraries.

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ?1 x 1010 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

Competent cell type Electrocompetent
Derivative of BL21 (DE3)
Species E. coli
Format Tubes
Transformation efficiency ?1 x 10^10 cfu/µg pUC19 DNA
Blue/white screening Yes
Shipping condition Dry ice

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