Cat #: 13-039
Intact Genomics 1232-24 ig® 5-alpha DH5, Electrocompetent Cells, 12 x 50µl/Unit
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Cat #: 13-039
Intact Genomics 1232-24 ig® 5-alpha DH5, Electrocompetent Cells, 12 x 50µl/Unit
Electrocompetent Cells
12 x 50µl/Unit
Brand: Intact Genomics- Suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning
- Contains ?2 x 1010cfu/µg efficiency with electroporation
- Greatly increase plasmid yield and quality due to endA1 mutation
- High-efficiency transformation with plasmids 30 kb in size
- Blue/white screening of recombinant clones due to lacZ?M15
- Ensured insert stability due to recA1 mutation
- Includes: ig 5-alpha electroCompetent cells, pUC19 control DNA, and recovery medium
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$265.00
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Electrocompetent Cells
12 x 50µl/Unit
Brand: Intact Genomics- Suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning
- Contains ?2 x 1010cfu/µg efficiency with electroporation
- Greatly increase plasmid yield and quality due to endA1 mutation
- High-efficiency transformation with plasmids 30 kb in size
- Blue/white screening of recombinant clones due to lacZ?M15
- Ensured insert stability due to recA1 mutation
- Includes: ig 5-alpha electroCompetent cells, pUC19 control DNA, and recovery medium
Intact Genomics 5-alpha electrocompetent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning. Mutations in endA1 and recA1 ensure increased plasmid yield and improved plasmid quality.
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ?2 x 1010 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
Competent cell type | Electrocompetent |
---|---|
Derivative of | DH5-alpha |
Species | E. coli |
Format | Tubes |
Transformation efficiency | ?2 x 1010 cfu/µg pUC19 DNA |
Blue/white screening | Yes |
Shipping condition | Dry ice |
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