Intact Genomics (ig®) DirectPlate-XL™ DH10B Competent cells offer simple, fast, and robust results for your large DNA transformation needs – directly clone large DNA up to >250kb! DirectPlate-XL™ DH10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their large DNA transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate-XL™ DH10B chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.  Using proprietary technology, these cells can be used for the transformation of extremely large DNA (i.e. >10kb up to >250kb).

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×108 – 109 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.