Cat #: 13-020

Intact Genomics 1071-12 HB101, Chemically Competent Cells, 6 x 100µl/Unit

Intact Genomics 1071-12 HB101, Chemically Competent Cells, 6 x 100
Intact Genomics 1071-12 HB101, Chemically Competent Cells, 6 x 100
Intact Genomics 1071-12 HB101, Chemically Competent Cells, 6 x 100

Cat #: 13-020

Intact Genomics 1071-12 HB101, Chemically Competent Cells, 6 x 100µl/Unit


Chemically Competent Cells

6 x 100µl/Unit

Brand: Intact Genomics

  • Suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning
  • Contains the recA13 mutation that minimizes recombination and helps insert stability
  • Carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes
  • Includes: ig™ JM109(DE3) competent cells, pUC19 control DNA, and recovery medium

Guest Price:
$99.00
List Price: $108.90
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Chemically Competent Cells

6 x 100µl/Unit

Brand: Intact Genomics

  • Suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning
  • Contains the recA13 mutation that minimizes recombination and helps insert stability
  • Carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes
  • Includes: ig™ JM109(DE3) competent cells, pUC19 control DNA, and recovery medium

Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening.

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×108 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

Competent cell type Chemically competent
Derivative of HB101
Species E. coli
Format Tubes
Transformation efficiency ?1 x 10^8 cfu/µg pUC19 DNA
Blue/white screening No
Shipping condition Dry ice

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