Cat #: 11-591B
Zymo Research D5451 RRHP 5-hmC Library Prep Kit, Zymo Research, 25 Preps/Unit
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Cat #: 11-591B
Zymo Research D5451 RRHP 5-hmC Library Prep Kit, Zymo Research, 25 Preps/Unit
Zymo Research
25 Preps/Unit
Brand: Zymo Research- Innovative library preparation for strand-specific mapping of 5-hmC in DNA
- Streamlined workflow accommodates low (=100 ng) DNA inputs.
- Libraries are ready for Next-Generation sequencing (Illumina-compatible).
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$2,109.05
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Zymo Research
25 Preps/Unit
Brand: Zymo Research- Innovative library preparation for strand-specific mapping of 5-hmC in DNA
- Streamlined workflow accommodates low (=100 ng) DNA inputs.
- Libraries are ready for Next-Generation sequencing (Illumina-compatible).
The RRHP 5-hmC Library Prep Kit is the first comprehensive solution for quantitative analysis of genome-wide 5-hmC positions at single base resolution. The Reduced Representation Hydroxymethylcytosine Profiling(RRHP) system is based on blocking MspI digestion by glucosylating 5-hmC within MspI recognition sites. Shown in the schematic below, fragments lacking glucosylated-5-hmC at the adapter-ligation junction will be cleaved and not amplified by PCR. Positive selection relies on fragments containing 5-hmC being successfully amplified and analyzed.
Sample sources: Input =100 ng intact genomic DNA. DNA can be in water, TE, or low salt buffer and should be free of enzymatic inhibitors.
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