Cat #: 11-315A
Zymo Research D2001 Yeast Plasmid Miniprep I, Zymoprep, 100 Preps /Unit
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Cat #: 11-315A
Zymo Research D2001 Yeast Plasmid Miniprep I, Zymoprep, 100 Preps /Unit
Zymoprep
100 Preps /Unit
Brand: Zymo Research- Simple procedures for plasmid rescue from yeast
- Ideal for low copy and hard-to-isolate plasmids
- For isolation of plasmid DNA for downstream applications such as PCR, transformation, hybridization, etc
- Compatibility: S. cerevisiae, C. albicans, S. pombe, and other fungi species sensitive to yeast lytic enzymatic digestion (Zymolyase)
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$146.80
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Zymoprep
100 Preps /Unit
Brand: Zymo Research- Simple procedures for plasmid rescue from yeast
- Ideal for low copy and hard-to-isolate plasmids
- For isolation of plasmid DNA for downstream applications such as PCR, transformation, hybridization, etc
- Compatibility: S. cerevisiae, C. albicans, S. pombe, and other fungi species sensitive to yeast lytic enzymatic digestion (Zymolyase)
The Zymoprep is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. There is no need for glass beads, phenol, or vortexing. Instead, plasmid DNA is reliably recovered from yeast cells whether colonies, patches on plates, or liquid cultures are sampled. Plasmid yields are typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures. This kit also works well with low copy number yeast plasmids. Recovered plasmid DNA is in TE buffer and can be used for E. coli transformation, Western blotting, PCR, etc.
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