• Apex Bioresearch Products 42-198C Apex Hot Start Master Mix, 2X, 1.5mM MgCl2 (Final), 3 x 50ml/Unit

Cat #: 42-198C

Apex Bioresearch Products 42-198C Apex Hot Start Master Mix, 2X, 1.5mM MgCl2 (Final), 3 x 50ml/Unit

Cat #: 42-198C

Apex Bioresearch Products 42-198C Apex Hot Start Master Mix, 2X, 1.5mM MgCl2 (Final), 3 x 50ml/Unit

1.5mM MgCl2 (Final)

6000 Reactions/Unit

Brand: Apex Bioresearch Products
  • Apex Hot Start Master Mix, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, high yield and high specificity
  • Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
  • Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
  • screening, GC-rich, multiplex and direct colony PCR
  • Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
  • BLUE version also available (42-145) with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
  • Mix is stable refrigerated (4ºC) for at least 6 months without risk
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List Price: $7,462.95
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1.5mM MgCl2 (Final)

6000 Reactions/Unit

Brand: Apex Bioresearch Products
  • Apex Hot Start Master Mix, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, high yield and high specificity
  • Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
  • Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
  • screening, GC-rich, multiplex and direct colony PCR
  • Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
  • BLUE version also available (42-145) with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
  • Mix is stable refrigerated (4ºC) for at least 6 months without risk

Apex Hot Start Master Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. Apex Hot Start DNA Polymerase is a modified form of Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Apex Hot Start DNA Polymerase, ammonium buffer system, dNTPs and magnesium chloride are present in Apex Hot Start Master Mix with Apex Buffer I. Each reaction requires 150ul of the 2.0X reaction mix.

For most standard applications Master Mix based on Ammonium Buffer I is the best option. It promotes robust amplification, high yield and high specificity. In certain cases Master Mix with Buffer II is the best option.

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