This one hour protocol Prometheus® ProSignal® Rapid ECL kit includes all critical reagents required for 10 mini blots (excluding primary antibodies, transfer buffer, gel and ladder ) and generates high sensitivity chemiluminescent Western blot data in just one hour! The provided nitrocellulose membrane sheets are ideal for both large and small MW proteins (<15kda-400kda) and the rapid blocking buffer is specially formulated to increase sensitivity while decreasing overall background. The unique HRP Conjugate buffer amplifies signal to increase sensitivity and minimizes the duration of primary and secondary antibody incubation to 30 minutes total. The wash solution contains a gentle, non-ionic detergent that rapidly removes non-specifically bound material from the membrane without disrupting the antibody-antigen complex. Lastly, ProSignal ECL substrate is included for sensitive, long-lasting and flexible detection using X-ray film or CCD camera.

Enjoy the convenience of a simple and straight forward protocol, hours time savings, and high quality results with increased reproducibility from bundled reagents designed to work in unison with one another.