Intact Genomics (ig®) ATCC15834 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes pRi15834 (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms.  ATCC15834 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of legumes, tobacco, variety of grasses and other plants.  ATCC15834 Agrobacterium rhizogenes strain contains pRi15834 agrobacterium-type Ri plasmid and displays resistance to Rifampicin.

Transformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ?1 x 105 CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.