Intact Genomics (ig®) EHA105 (pSoup) ElectroCompetent Agrobacterium cells are optimized for the highest transformation efficiencies ideal for applications with cDNA or gDNA library construction. EHA105 (pSoup) contains a rifampicin resistance gene (rif).  EHA105 (pSoup) strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which contains the vir gene. The pSoup plasmid aids in replication of pGreen, 62SK, and pGs2 series plasmids. This system is used for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.

Transformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ?1 x 107 CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.