Intact Genomics Agrobacterium tumefaciens AGL1 (AGL-1) cells are optimized for the highest transformation efficiencies which are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.

The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicillin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for the transfer of genetic material into a host plant’s genome. Therefore, this system is often used for the Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.