Intact Genomics (ig®) GV3101 (pSoup-p19) Electro-Competent Agrobacterium cells are optimized for the highest transformation efficiencies, ideal for applications with cDNA or gDNA library construction.  The GV3101 (pSoup-p19) strain has resistance to rifampicin, gentamicin, and tetracyline (see genotype). The GV3101 Ti plasmid has the T-DNA region deleted and transformation with a binary vector containing the missing T-region restores the T-DNA binary system function. The pSoup plasmid aids in replication of pGreen, 62SK, and pGs2 series plasmids. Addition of p19 inhibits RNA silencing of foreign genes, resulting in improved stability of heterologous gene transcripts. This system is used for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.

Transformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ?1 x 107 CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.