Cat #: 13-022
Intact Genomics 1076-12 MG1655, Chemically Competent Cells, 12 x 50µl/Unit
Cat #: 13-022
Intact Genomics 1076-12 MG1655, Chemically Competent Cells, 12 x 50µl/Unit
Chemically Competent Cells
12 x 50µl/Unit
Brand: Intact Genomics- Suitable for routine DNA transformation and other research purposes
- Cured of the temperate bacteriophage lambda and F plasmid
- Possesses a frameshift at the end of rph
- Contains ilvG- mutation that is a frameshift which knocks out acetohydroxy acid synthase II
- Incorperates a rfb-50 mutation which is an IS5 insertion that results in the absence of O-antigen synthesis
- Includes: ig® MG1655 competent cells, pUC19 control DNA, and recovery medium
$190.00
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Chemically Competent Cells
12 x 50µl/Unit
Brand: Intact Genomics- Suitable for routine DNA transformation and other research purposes
- Cured of the temperate bacteriophage lambda and F plasmid
- Possesses a frameshift at the end of rph
- Contains ilvG- mutation that is a frameshift which knocks out acetohydroxy acid synthase II
- Incorperates a rfb-50 mutation which is an IS5 insertion that results in the absence of O-antigen synthesis
- Includes: ig® MG1655 competent cells, pUC19 control DNA, and recovery medium
Intact Genomics (ig®) chemically competent MG1655 chemically competent cells are suitable for routine DNA transformation and other research purposes. MG1655 is the “wild type” K-12 strain with minimal genetic manipulation having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange (1).
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high efficiency transformation protocol. Transformation efficiency should be greater than 1×109 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
| Competent cell type | Chemically competent |
|---|---|
| Derivative of | MG1655 |
| Species | E. coli |
| Format | Tubes |
| Transformation efficiency | ?1 x 10^9 cfu/µg pUC19 DNA |
| Blue/white screening | No |
| Shipping condition | Dry ice |
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