Cat #: 11-698B

Zymo Research D5013-1 Human WGA Non-Methylated DNA, Zymo Research, (5µg/20µl)/Unit

Zymo Research D5013-1 Human WGA Non-Methylated DNA, Zymo Research, (5
Zymo Research D5013-1 Human WGA Non-Methylated DNA, Zymo Research, (5
Zymo Research D5013-1 Human WGA Non-Methylated DNA, Zymo Research, (5

Cat #: 11-698B

Zymo Research D5013-1 Human WGA Non-Methylated DNA, Zymo Research, (5µg/20µl)/Unit


Zymo Research

(5µg/20µl)/Unit

Brand: Zymo Research

  • Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications
  • Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency
  • Primers designed to amplify non-methylated, methylated, and mixed methylated Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene

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Zymo Research

(5µg/20µl)/Unit

Brand: Zymo Research

  • Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications
  • Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency
  • Primers designed to amplify non-methylated, methylated, and mixed methylated Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene

The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.

Brand Zymo Research Corporation
Concentration 250ng/µl in buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0)
Sample Source Whole genome amplified DNA from HCT116 DKO cells. Human WGA Methylated DNA is enzymatically methylated by M.SssI methyltransferase.
Sample Storage </= -20°C

All standards are enzymatically methylated by CpG Methylase in vitro at all CpG dinucleotides. The methylated DNA is very highly methylated (> 95% of all CpG sites).

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