Cat #: 11-698
Zymo Research D5013 Human Methylated & Non-Methylated (WGA) DNA Set, Zymo Research, (5µg/20µl)/Unit
Cat #: 11-698
Zymo Research D5013 Human Methylated & Non-Methylated (WGA) DNA Set, Zymo Research, (5µg/20µl)/Unit
Zymo Research
(5µg/20µl)/Unit
Brand: Zymo Research- Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications
- Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency
- Primers designed to amplify non-methylated, methylated, and mixed methylated Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene
- Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
$439.65
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Zymo Research
(5µg/20µl)/Unit
Brand: Zymo Research- Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications
- Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency
- Primers designed to amplify non-methylated, methylated, and mixed methylated Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene
- Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.
| Brand | Zymo Research Corporation |
|---|---|
| Concentration | 250ng/µl in buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0) |
| Sample Source | Whole genome amplified DNA from HCT116 DKO cells. Human WGA Methylated DNA is enzymatically methylated by M.SssI methyltransferase. |
| Sample Storage | </= -20°C |
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Q1: Are the Human methylated DNA standards completely (100%) methylated at the CpG sites?
All standards are enzymatically methylated by CpG Methylase in vitro at all CpG dinucleotides. The methylated DNA is very highly methylated (> 95% of all CpG sites).
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