• Zymo Research D5463 Zymo-Seq Cell Free DNA WGBS Library Kit, Zymo Research, 96 Preps/Unit

Cat #: 11-618B

Zymo Research D5463 Zymo-Seq Cell Free DNA WGBS Library Kit, Zymo Research, 96 Preps/Unit

Zymo Research D5463 Zymo-Seq Cell Free DNA WGBS Library Kit, Zymo Research, 96 Preps/Unit Primary Image
Zymo Research D5463 Zymo-Seq Cell Free DNA WGBS Library Kit, Zymo Research, 96 Preps/Unit Primary Image
Zymo Research D5463 Zymo-Seq Cell Free DNA WGBS Library Kit, Zymo Research, 96 Preps/Unit Primary Image

Cat #: 11-618B

Zymo Research D5463 Zymo-Seq Cell Free DNA WGBS Library Kit, Zymo Research, 96 Preps/Unit

Zymo Research

96 Preps/Unit

Brand: Zymo Research
  • Optimized for small fragment input
  • Direct ligation-based protocol allows for accurate methylation calling
  • Prepare robust methyl-seq libraries in as little as 3 steps
Guest Price:
$4,801.50
List Price: $4,950.00
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Zymo Research

96 Preps/Unit

Brand: Zymo Research
  • Optimized for small fragment input
  • Direct ligation-based protocol allows for accurate methylation calling
  • Prepare robust methyl-seq libraries in as little as 3 steps

Zymo-Seq Cell Free DNA WGBS Library Kit provides an optimized and reliable workflow for whole genome bisulfite sequencing (WGBS) library preparation from cell-free DNA (cfDNA). This all-inclusive kit features a straightforward procedure capable of preparing high-quality methyl-seq libraries from as little as 5 ng of cfDNA. The process is completed in three basic steps: bisulfite conversion, direct adapter ligation, and index PCR amplification.

Bisulfite Conversion ?99.5% of non-methyllated cytosine residues are converted to uracil; ?99.5% protecton of methylated cytosines.
Equipment Required Thermal cycler(s) with temperature adjustable lids, microcentrifuge, and magnetic stand
Hands-On Time ?2 hours
Total Processing Time ?6 hours
Input Quality For optimal results, use at least minimum input of purified cfDNA with no RNA or gDNA contamination. cfDNA can be concentrated using the DNA Clean & Concentrator (D4013) prior to processing. Input of cfDNA can be suspended in water, DNA Elution Buffer, or TE buffer.
Library Storage Libraries eluted in DNA Elution Buffer (provided) may be stored at ?4°C overnight or ?-20°C for long-term storage.
Minimum Input 5 ng
Maximum Input 10 ng
Sample Input Material Purified cell-free DNA (cfDNA)
Sequencing Compatibility Libraries are compatible with all Illumina sequencing platforms. Recommended: NextSeq, NovaSeq.

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