The OneStep qMethyl Kit provides a simple, straightforward, and bisulfite-free procedure for rapid, locus-specific DNA methylation assessment via the selective amplification of a methylated region of DNA. This is accomplished by splitting any DNA to be tested into two parts: a “Test Reaction” and a “Reference Reaction”. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both samples is then amplified using real-time PCR in the presence of SYTO 9 fluorescent dye and quantitated using a simple equation.