The Quick-DNA™ FFPE Kit provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using the provided Proteinase K, heat, and then purify the DNA with the Fast-Spin columns in the kit. DNA >50 bp or >500 bp can be selectively isolated by altering the lysis buffer conditions as given in the protocol. PCR inhibitors are effectively removed during the isolation procedure, and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc.

Sample Size: Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (=20 µm thick) with a total surface area ~20 cm2 . It is recommended to use 1-2 sections if performing the protocol for the first time. Compatible with fresh/frozen tissue specimens