The cDNA Synthesis Kit utilizes the Ultrascript Reverse Transcriptase (also sold separately), which is a modified MMLV Rtase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.

The UltraScript RTase allows for reaction temperatures up to 55°C. This higher incubation temperature allows for increased sensitivity and generation of more cDNA from templates with high GC %.

The cDNA syntheis kit is a simple, two-tube system. One tube of 20x RTase with RNase inhibitor and one tube of 5x cDNA synthesis mix that contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl2.

The cDNA synthesis mix can be used with as little as 4pg and up to 0.4ug total RNA. The Universal primers and random hexamers can be used with all types of RNA including viral RNA targets, miRNA targets, poly(A)+ RNA, etc.

The Ultrascript 2.0 Reverse Transcriptase (sold separately on in a cDNA synthesis kit) is a modified MMLV Rtase engineered for superior cDNA synthesis speed and yield on the most challenging RNA sample types. It comes blended with an advanced RNase inhibitor to prevent degration by RNase.

The UltraScript 2.0 RTase is highly thermostable at 55°C to 65°C and above. The enzyme remains partially active even up to 90°C and is designed for efficient reverse transcription of the most difficult RNA templates, including GC-rich and highly structured transcripts.

UltraScript 2.0 RTase is available in three convenient formats, each with dNTPs, MgCl2, and RNase inhibitor included. It is available as a standalone enzyme, in a cDNA synthesis kit blended with optimized oligo(dT) and random hexamers or as a cDNA synthesis kit with separate Oligos

The UltraScript 2.0 kits can be used with a broad range of RNA concentrations. They can be used with 20pg to 3.5ug total RNA or oligo(dT) purified mRNA. The universal primers and random hexamers can be used with all types of RNA including viral RNA targets, miRNA targets, poly(A)+ RNA, etc.