Real-Time PCR SyGreen Mixes

Highly Specific

Market leading sensitivity that outperforms competitors even on complex templates including GC and AT-rich sequences

Extremely Fast

Rapid extension rates lead to early Ct values. Industry-leading performance in both fast and ultra fast cycling conditions

Optional Blue Dye

Optional non-reactive blue dye allows for direct-to-gel analysis and easy visualization during pipetting

Antibody Mediated

Features and antibody-mediated hot start with ~2 min. activation step

Proprietary SyGreen Dye

Uses a proprietary green dye that doesn’t inhibit PCR like other popular fluorescent dyes

Universally Compatible

Compatible for use on all real-time PCR platforms. Click here for instrument compatibility


SHOP NOW




Real-Time PCR Probe Mixes

Universal Compatibility

Superior performance in all probe-based qPCR assays, including TaqMan®, Scorpions® and molecular beacon probes

Multiplex Reactions

Proven high efficiency for reliable performance in multiplex expression studies

Optional Blue Dye

Optional non-reactive blue dye allows for direct-to-gel analysis and easy visualization during pipetting

Antibody Mediated

Features and antibody-mediated hot start with ~2 min. activation step


SHOP NOW





Not Sure Which ROX Level is Right for You? Click Here for Our qPCR Selection Table for Instrument Compatibility!


REAL-TIME PCR HRM
(HIGH RESOLUTION MELT) MIX

The HRM mix combines the latest developments in polymerase technology and advanced buffer chemistry to provide reliable and accurate detection of genetic mutations and SNPs using High Resolution Melting (HRM) analysis following qPCR with minimal or no optimization.

• Fast and accurate detection of subtle sequence differences
• Antibody-mediated hot start technology for the best sensitivity
• Compatible with all real-time instruments with HRM capability


Not Sure Which ROX Level is Right for You? Click Here for Our qPCR Selection Table for Instrument Compatibility!
The HRM Mix produces ultra-sensitive melt profiles allowing for accurate discrimination of subtle sequence variations for class I to class IV SNP mutations




HRM Resources/Documents

HRM Protocol > HRM Product Insert >



REAL-TIME PCR GENOTYPING MIX

The qPCR Genotyping Mix is for use in TaqMan and other dual-labelled probe-based genotyping assays. The mix is compatible with LNA and PNA probes, which offer more stringent allele calling. The hot start technology gives improved reaction sensitivity and excellent room temperature stability, ideal for high-throughput genotyping studies.

• Fast, accurate and reliable mix for reproducible allelic discrimination
• Antibody-mediated hot start technology for the best sensitivity
• Superior allele clustering and accurate characterization of class I to class IV mutations


Not Sure Which ROX Level is Right for You? Click Here for Our qPCR Selection Table for Instrument Compatibility!
Each SNP was analyzed using TaqMan® probes and our Genotyping Mix. All SNPs demonstrate high confidence, clear clustering of homozygous wt, homozygous mutant and heterozygous.



RT-QPCR SYGREEN 1-STEP KITS

cDNA Synthesis and RT-qPCR in One Single Tube!

SyGreen 1-Step GoDesigned for speed (earliest Ct) and is ideally suited to detection
from high template concentrations. Recommended for template amounts of 10pg – 100ng total RNA or >0.01pg mRNA per reaction.

SyGreen 1-Step Detect
Designed for sensitivity and is ideally suited to the detection of extremely low copy number targets. Recommended for template amounts of 1pg – 10ng total RNA or >0.01pg mRNA per reaction.


Not Sure Which ROX Level is Right for You? Click Here for Our qPCR Selection Table for Instrument Compatibility!
The ACTG1 gene was amplified from a dilution series of total RNA extracted from mouse liver. qPCRBIO SyGreen 1-Step Go (pink) shows efficient amplification of total RNA in the range 100pg – 100ng per reaction. qPCRBIO SyGreen 1-Step Detect (blue) shows efficient amplification of total RNA in the range 10pg – 10ng per reaction.



RT-QPCR PROBE 1-STEP GO KITS

cDNA Synthesis and probe-based RT-qPCR in One Tube!
The Probe 1-Step Go kit is a universal probe kit designed for fast, highly specific and ultra-sensitive probe-based RT-qPCR. The kit is engineered for use on a wide range of probe technologies including TaqMan®, Scorpions® and molecular beacon probes.

• High efficiency in multiplex reactions
• Thermostable reverse transcriptase 45°C to 55°C
• Antibody-mediated hot start PCR


Not Sure Which ROX Level is Right for You? Click Here for Our qPCR Selection Table for Instrument Compatibility!
qPCRBIO Probe 1-Step Go mix shows high efficiency over a broad dynamic range