For some applications such as cloning and site directed mutagenesis, or when preparing a template for sequencing, the number of mutations introduced during PCR needs to be kept to a minimum. The enhanced accuracy of HS VeriFi Polymerase gives extremely low error rates, making it ideal for such applications where superior accuracy is required.
VeriFi polymerase is a high fidelity enzyme derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The versatile and robust proofreading enzyme is able to amplify longer and more difficult targets such as eukaryotic genomic templates up to 17.5kb, and longer for simpler DNA templates. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.
HOT START VERIFI™ POLYMERASE & MIXES
Our most versatile proofreading enzyme now with AptaLock™ hot start technology for maximised specificity in your high fidelity PCR. Ideal for multiplex reactions and room-temperature setup.
Our highest fidelity enzyme, ideal for cloning, sequencing and site-directed mutagenesis. Get better, more consistent results, even with long and difficult templates.
