Proteinase K is a protease that cleaves at the carboxyl side of aliphatic, aromatic or hydrophobic residues and is commonly used to digest and inactivate DNase and RNase in nucleic acid purification. Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungusTritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. The activity of proteinase K is increased at elevated temperatures up to 65°C. Calcium is not essential to the function of proteinase K. Therefore, EDTA and other chelating agents do not interfere with the activity and may be used alongside proteinase K to inactivate calcium dependent nucleases in DNA and RNA preparation.

Gentrox brand products are produced in manufacturing facilities that feature state-of-theart automation and are certified to ISO 2001:2008. Automated buffer production and filling facilities are designed to produce bulk chemicals as well as smaller custom batches. At Gentrox, we understand that our products can be used in regulated environments and therefore must meet exacting quality standards. Our manufacturing facilities have a quality system that is designed for cGMP compliance and ensures that all products are made to required specifications.