GC5 Mix & Plate Competent Cells
Cat #: 42-652MG
10^8 - 10^9 transformants/µg
10 x 100ul/Unit
- No heat shock, no lengthy incubations, no outgrowth procedures, no wait!
- Simple procedure: add DNA and then spread. DNA transformation in as little as 20 seconds!
- Mix & Plate! transformation procedure will work for most transformations using Ampicillin selection and not requiring outgrowth.
- Insert stability due to Reca1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation
- High plasmid yield due to endA1 mutation
- For general cloning, blue-white selection, plasmid isolation
- Transformation efficiencies of 10^8 - 10^9 transformants/µg of plasmid DNA
- F-f80lacZ?M15 ?(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1, ?-
DescriptionThe GC5 Mix & Plate E. Coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. These cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium - Mix & Plate! The premade cells are highly efficient (> 10^8 transformants / µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc. Supplied as a pack of 10 convenient 100 µl/tube single use aliquots or in a 96-tube format with removable 8-tube strips for your high-throughput transformation needs.
The Mix & Plate procedure will work for most transformations using Ampicillin selection and not requiring outgrowth. The highest transformation efficiencies can be obtained by incubating the cells with DNA on ice for 2-5 minutes (60 minutes maximum) prior to plating. When selecting with Kanamycin, Tetracycline, etc., an outgrowth performed in SOC medium is required for efficient transformation. In most cases, this step can be omitted when selecting with Ampicillin.