Cat #: 20-511

Prometheus Protein Biology Products 20-511 Cobalt Metal Affinity Resin, Cross-linked Beads, 6%, 100ml/Unit

Prometheus Protein Biology Products 20-511 Cobalt Metal Affinity Resin, Cross-linked Beads, 6%, 100ml/Unit primary image
Prometheus Protein Biology Products 20-511 Cobalt Metal Affinity Resin, Cross-linked Beads, 6%, 100ml/Unit primary image
Prometheus Protein Biology Products 20-511 Cobalt Metal Affinity Resin, Cross-linked Beads, 6%, 100ml/Unit primary image

Cat #: 20-511

Prometheus Protein Biology Products 20-511 Cobalt Metal Affinity Resin, Cross-linked Beads, 6%, 100ml/Unit


Cross-linked Beads, 6%

100ml/Unit

Brand: Prometheus Protein Biology Products

  • Ready-to-use IMAC resin that utilizes nickel (Co2 ) for purifying 6x His-tagged proteins
  • High selectivity: appropriate for valuable recombinant proteins in limited quantities
  • Allows for purification of proteins under native or denaturing conditions
  • High capacity: >50mg protein per ml resin
  • Ligand density: 20-40umol Ni2 /ml resin
  • Bead specifications: Spherical, ~50 - 150um
  • Available in bulk or in prepacked 1ml or 5ml gravity flow columns
  • For batch or column purifications

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$888.10
List Price: $1,144.20
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Cross-linked Beads, 6%

100ml/Unit

Brand: Prometheus Protein Biology Products

  • Ready-to-use IMAC resin that utilizes nickel (Co2 ) for purifying 6x His-tagged proteins
  • High selectivity: appropriate for valuable recombinant proteins in limited quantities
  • Allows for purification of proteins under native or denaturing conditions
  • High capacity: >50mg protein per ml resin
  • Ligand density: 20-40umol Ni2 /ml resin
  • Bead specifications: Spherical, ~50 - 150um
  • Available in bulk or in prepacked 1ml or 5ml gravity flow columns
  • For batch or column purifications

Prometheus™ Co-IDA Agarose resin is an affinity chromatography resin for purification of histidine-tagged proteins by Immobilized Metal Affinity Chromatography (IMAC). It binds to six histidine residues (6x His), a common tag used in protein purification and consists of iminodiacetate (IDA - a tridentate chelating agent) coupled to 6% cross-linked agarose beads. The IDA binds divalent cobalt ion with a capacity of 20-40umoles Co2+/ml resin. The protein binding capacity is >50mg protein per ml resin, but we have demonstrated binding of >130mg of purified DHAK-6XHis tagged protein to a ml of resin.

Although His-tagged proteins bind to Co-IDA with a slightly lower efficiency compared to Ni-IDA Agarose, there is a significant reduction in non-specific binding. Cobalt resins offer higher selectivity for poly-His sequences, but they have a low loading capacity. Therefore Cobalt Chelating Resin should be used for valuable recombinant proteins in limited quantities. The purity and yield of the target protein varies and is dependent on many factors including working conditions, size, conformation, expression levels, solubility and sample impurities.

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