Cat #: 17-603

PCR Biosystems PB25.32-01 qPCRBIO SyGreen 1-Step Go Hi-ROX, 1 x 1ml Mix & 1 x 100ul RTase, 100 x 20ul Rxns/Unit

Cat #: 17-603

PCR Biosystems PB25.32-01 qPCRBIO SyGreen 1-Step Go Hi-ROX, 1 x 1ml Mix & 1 x 100ul RTase, 100 x 20ul Rxns/Unit


1 x 1ml Mix & 1 x 100ul RTase

100 x 20ul Rxns/Unit

Brand: PCR Biosystems

  • Designed for speed (earliest Ct) and ideally suited to detection from high template concentrations
  • Recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction
  • Thermostable reverse transcriptase 45°C to 55°C
  • Contains advanced RNase inhibitor
  • Non-PCR inhibiting intercalating dye
  • Rapid extension rate for early Ct values
  • Market leading sensitivity - increased limit of detection
  • Compatible on all real-time PCR platforms - standard and fast cycling conditions

Kit Type

Rox Level

Size


Guest Price:
$172.00
List Price: $227.80
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1 x 1ml Mix & 1 x 100ul RTase

100 x 20ul Rxns/Unit

Brand: PCR Biosystems

  • Designed for speed (earliest Ct) and ideally suited to detection from high template concentrations
  • Recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction
  • Thermostable reverse transcriptase 45°C to 55°C
  • Contains advanced RNase inhibitor
  • Non-PCR inhibiting intercalating dye
  • Rapid extension rate for early Ct values
  • Market leading sensitivity - increased limit of detection
  • Compatible on all real-time PCR platforms - standard and fast cycling conditions

qPCRBIO 1-Step SyGreen mix uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by PCRBIO Taq HS DNA Polymerase, resulting in robust RT-PCR performance. The 1-step kits can be used to quantify any RNA template inclusing mRNA, total RNA and viral RNA sequences. The 1-step Go kit is designed for extremely fast qPCR and gives the earliest Ct values from high template concentrations.

qPCRBIO 1-Step SyGreen mix can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation. any modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.

Reactions 100 x 20ul Rxns/Unit
Packaging 1 x 1ml Mix & 1 x 100ul RTase
Storage Between -30 and -15°C
Recommended Template Amount 10pg - 100ng total RNA or >0.01pg mRNA per reaction
Compare To BIORAD iTaq™ Universal SYBR® Green One-Step Kit, Qiagen QuantiFast SYBR® Green RT-PCR Kit, NEB Luna® Universal One-Step RT-qPCR Kit, Promega GoTaq® 1-Step RTqPCR, Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG

No, this kit will not work for 2-step reactions. For 2-step reactions, we recommend the qPCRBIO cDNA Synthesis Kit or UltraScript 2.0 cDNA Synthesis Kit for more difficult targets, along with real time PCR kits depending on your needs.

No, the RTase Go contains an RNase inhibitor to prevent any degradation and increase sensitivity.

qPCRBIO SyGreen 1-step Detect has been engineered to have high sensitivity for rapid and accurate results from low template concentrations 1pg of total RNA or 0.01pg of mRNA. qPCRBIO SyGreen 1-step Go has been developed for rapid (early Ct) and accurate results from high template concentrations from 10pg total RNA or 0.01pg of mRNA.

Yes, qPCRBIO SyGreen 1-Step Detect | Go can be used for micro RNA templates. Although we do no sell dedicated kits, all of our RTases can be used for miRNA quantification and analysis. Please reach out if you'd like recommended protocols.

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