Cat #: 17-301B

PCR Biosystems PB15.11-24 PCRBIO Rapid Extract Lysis Kit, Extract DNA from Mammalian Tissue, 240 Reactions/Unit

PCR Biosystems PB15.11-24 PCRBIO Rapid Extract Lysis Kit, Extract DNA from Mammalian Tissue, 240 Reactions/Unit primary image
PCR Biosystems PB15.11-24 PCRBIO Rapid Extract Lysis Kit, Extract DNA from Mammalian Tissue, 240 Reactions/Unit secondary image
PCR Biosystems PB15.11-24 PCRBIO Rapid Extract Lysis Kit, Extract DNA from Mammalian Tissue, 240 Reactions/Unit primary image
PCR Biosystems PB15.11-24 PCRBIO Rapid Extract Lysis Kit, Extract DNA from Mammalian Tissue, 240 Reactions/Unit primary image
PCR Biosystems PB15.11-24 PCRBIO Rapid Extract Lysis Kit, Extract DNA from Mammalian Tissue, 240 Reactions/Unit secondary image

Cat #: 17-301B

PCR Biosystems PB15.11-24 PCRBIO Rapid Extract Lysis Kit, Extract DNA from Mammalian Tissue, 240 Reactions/Unit


Extract DNA from Mammalian Tissue

240 Reactions/Unit

Brand: PCR Biosystems

  • Rapid, convenient, single-tube DNA extraction
  • Produces high yield, PCR ready DNA in 15 minutes
  • Maximized PCR speed, yield and specificity
  • Minimized contamination risks and sample loss
  • Also available as a full PCR Extraction kit that includes our Hot Start Taq Polymerase (Cat #:17-207)

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$358.35
List Price: $516.30
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Extract DNA from Mammalian Tissue

240 Reactions/Unit

Brand: PCR Biosystems

  • Rapid, convenient, single-tube DNA extraction
  • Produces high yield, PCR ready DNA in 15 minutes
  • Maximized PCR speed, yield and specificity
  • Minimized contamination risks and sample loss
  • Also available as a full PCR Extraction kit that includes our Hot Start Taq Polymerase (Cat #:17-207)

PCRBIO Rapid Extract Lysis Kit from Genesee Scientific has been developed for fast, efficient extraction of DNA from a variety of tissues and is particularly suited to solid tissue such as mouse tail or mouse ear. Sample processing is simplified and contamination risks minimized as DNA extraction is performed in a single tube, removing the need for multiple washing steps. 

PCRBIO Rapid Extract Lysis Kit contains only the lysis and protease buffer system allowing the generation of PCR-ready DNA for use in downstream PCR or qPCR reactions.

Packaging 3 x 1.6ml Buffer A and 3 x 800ul Buffer B & 10
Reactions 240 x 50ul Rxns/Unit
Storage Between -30 and -15°C
Compare To Sigma Extract-N-Amp™ Tissue PCR Kit, KAPA Mouse Genotyping Kit

Our PCR mixes work reliably with 10 or more copies of template per reaction but not lower than that. Keep the extracted DNA in the lowest volume possible and don’t dilute further after inactivating the protease. We advise setting up the assay with more cells and once the conditions are established, scaling it down from there.

Yes, it can be used to amplify DNA from bacterial colonies or bacterial cell lysate however, it’s not necessary to use the Rapid Extract PCR Kit in order to perform routine colony PCR. If you’re working from bacterial colonies use a sterile tip to pick a colony and resuspend into the 50µl PCR reaction. If working from liquid culture add 5µl of overnight culture to the final mix. Follow the general protocol and increase the initial denaturation time to 10 min at 95°C. For qPCR, it may be important to extract DNA with the PCRBIO Rapid Extract Lysis Kit to decrease background noise and control the amount of DNA added to the reaction.

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