(55-816JB) CD16 mFluor™ 450 Antibody Clone 3G8, Mouse IgG1,k 100 Tests/Unit
(55-816JB) CD16 mFluor™ 450 Antibody Clone 3G8, Mouse IgG1,k 100 Tests/Unit
(55-816JB) CD16 mFluor™ 450 Antibody Clone 3G8, Mouse IgG1,k 100 Tests/Unit
(55-816JB) CD16 mFluor™ 450 Antibody Clone 3G8, Mouse IgG1,k 100 Tests/Unit
(55-816JB) CD16 mFluor™ 450 Antibody Clone 3G8, Mouse IgG1,k 100 Tests/Unit
(55-816JB) CD16 mFluor™ 450 Antibody Clone 3G8, Mouse IgG1,k 100 Tests/Unit
CAT#: 55-816JB Caprico Biotechnologies

CD16 mFluor™ 450 Antibody Clone 3G8

Clone 3G8, Mouse IgG1,k, 100 Tests/Unit

List Price: $596.35
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Product Overview
  • Monoclonal antibody specific to human and non-human primate CD16
  • The 3G8 antibody has been reported for use in flow cytometric analysis and functional assays
  • Utilizes mFluor™ fluorescent labeling for greater light stability and brighter fluorescence
  • Highly specific and little to no cross reactivity to ensure accurate, reproducible results in Flow Cytometry
  • >90% pure tested via polyacrylamide gel electrophoresis (PAGE)
  • More conjugations and unit sizes available. Contact us at support@geneseesci.com to inquire.
Technical Specifications
ConjugationmFluor™450
Excitation LaserViolet (405nm)
ReactivityHuman, Rhesus, Cynomolgus monkey, Baboon
HostMouse
AntigenCD16
Clone3G8
IsotypeIgG1,k
StatusRUO
Size100 Tests/Unit
Storage2-8°C and protected from prolonged exposure to light
FormulationPBS pH 7.2, 0.2% BSA (w/v), 0.09% sodium azide
ImmunogenHuman peripheral blood T lymphocytes
ClonalityMonoclonal
Detailed Description
The clone 3G8, a mouse monoclonal antibody, binds to the 50-65 kDa transmembrane protein of IgG Fc?RIII known as CD16, a surface antigen commonly associated with human NK cells. In addition to surface expression on NK cells, CD16 is expressed on activated monocytes, macrophages, neutrophils, and placental trophoblasts in humans. 3G8 binds either with the aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell mediated cytotoxicity (ADCC). Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.

mFluor™ fluorescent labeling dyes are developed specifically for flow cytometry applications to enable optimal multi-color detection. These dyes are excellent alternatives to the phycoprotein-based tandems that are quite difficult to couple to an antibody or other biomolecules and offer greater light stability and brighter fluorescence. The mFluor™ 450 fluorophore is a great alternative to these dyes: V450, Pacific Blue™, VioBlue®, eFluor® 450, Brilliant Violet 421 and Super Bright 436. Each lot of these antibodies has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 0.5µg (unconjugated, Biotin) or 5µl (conjugated) of these products are sufficient for staining 1 million cells in a 100µl staining volume or 100µl of whole blood. It is recommended to titrate antibody reactivity empirically for optimal performance. Non-human primate cross-reactivity has been validated using the APC conjugated clone 3G8 product. Do you need an antibody for an antigen you don't see on our website? Do you need a different conjugation than what we have listed? We have a wide selection of antigens with a variety of conjugations available. Please reach out to us at support@geneseesci.com.
Citations

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