Cat #: 55-312
Polyplus-Transfection 101000026 PEIpro® DNA Transfection Reagent, Large-Scale Virus Production, 100ml/Unit
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Cat #: 55-312
Polyplus-Transfection 101000026 PEIpro® DNA Transfection Reagent, Large-Scale Virus Production, 100ml/Unit
Large-Scale Virus Production
100ml/Unit
Brand: Polyplus-transfection- Optimized PEI-based transfection reagent for scalable virus production
- Superior viral titers compared to other commercial PEIs and Calcium Phosphate
- Suitable for Process Development of large-scale virus production
- Reliable and secure supply
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Size
$7,257.00
Large-Scale Virus Production
100ml/Unit
Brand: Polyplus-transfection- Optimized PEI-based transfection reagent for scalable virus production
- Superior viral titers compared to other commercial PEIs and Calcium Phosphate
- Suitable for Process Development of large-scale virus production
- Reliable and secure supply
PEIpro® transfection reagent is the leading chemical-based DNA transfection reagent that offers flexibility and scalability to develop a robust and sustainable Process Development for viral vector production. PEIpro® benefits from extensive research development in PEI polymer chemistry and formulation to achieve highest transfection efficiency in both adherent and suspension cell culture systems. Protocols established with PEIpro® during Process Development are directly implementable for large-scale manufacturing of preclinical and clinical batches of viral vectors with the higher quality grades of PEIpro®: PEIpro®-HQ and PEIpro®-GMP.
With its unique property to efficiently condense several plasmids of different sizes, PEIpro® transfection reagent is ideal for optimal co-transfection of several plasmids containing the gene of interest and necessary viral components to produce full recombinant virions. PEIpro® has benefited from extensive developments that made it the unique PEI-based transfection reagent that can offer the flexibility and robustness that is needed during Process Development and Scale-up; it is compatible with commercially available adherent and suspension virus production systems for lentivirus and AAV viral vectors
Brand | Polyplus-Transfection |
---|---|
Reagent Volume | 100ml |
Sterile | Yes |
Concentration | 1 mg/ml |
Molecule Delivered | Plasmid DNA and co-delivery of several plasmid DNA |
Applications | Large-scale virus production |
Virus Types | Mainly adenovirus, Adeno-Associated Viruses (AAV), Lentivirus, influenza virus, retrovirus, Virus-like particles (VLP) |
Cell Types | Mammalian producer cell lines (HEK-293, HEK-293 derivatives, BHK, VERO cell lines, virus-specific packaging cell lines. |
Number of Transfections | 100ml of PEIpro® transfection reagent is sufficient to transfect on average 50 L of cell culture |
Storage Condition | 5 ± 3°C |
Shipping Condition | Ambient |
Shelf Life | 36 months from manufacturing date |
Provided with | Protocol, certificate of analysis, certificate of origin and non-hazardous product statement |
PEIpro® is composed of a linear polyethylenimine (PEI) whose molecular weight is proprietary information. Extensive optimization experiments have been performed to select a linear PEI for optimal DNA delivery with the lowest cytotoxicity in vitro. PEIpro® is provided as an aqueous solution of highly pure and qualified linear PEI at a concentration of 1 mg/L.
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