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Apex Bioresearch Products
Apex 5X cDNA Synthesis Supermix
First-strand cDNA synthesis, 500 x 20ul Reactions/Unit
First-strand cDNA synthesis, 50 x 20ul Reactions/Unit
First-strand cDNA synthesis, 100 x 20ul Reactions/Unit
3 product variations: View Options
Product Overview
- Simple, fast, and sensitive first-strand cDNA synthesis kit for RT-qPCR or RT-PCR
- Preblended 5X supermix contains all the necessary components except RNA template for reverse transcription
- Enhanced, engineered MMLV Rtase has reduced RNase H activity and contains a fidelity-enhancing subunit for superior accuracy
- Potent blend of RNase inhibitor prevents RNA degradation during reaction setup and reverse transcription
- Contains optimized blend of oligo(dT) and random primers for complete RNA coverage
- Complete cDNA synthesis and PCR the same day with 15 minute (for qPCR) or 50 minute (for PCR) cDNA synthesis protocol
- Both poly(A) + mRNA and total RNA can be used for first-strand cDNA synthesis, but poly(A) + mRNA may give higher yields and improved purity
Product Overview
- Simple, fast, and sensitive first-strand cDNA synthesis kit for RT-qPCR or RT-PCR
- Preblended 5X supermix contains all the necessary components except RNA template for reverse transcription
- Enhanced, engineered MMLV Rtase has reduced RNase H activity and contains a fidelity-enhancing subunit for superior accuracy
- Potent blend of RNase inhibitor prevents RNA degradation during reaction setup and reverse transcription
- Contains optimized blend of oligo(dT) and random primers for complete RNA coverage
- Complete cDNA synthesis and PCR the same day with 15 minute (for qPCR) or 50 minute (for PCR) cDNA synthesis protocol
- Both poly(A) + mRNA and total RNA can be used for first?strand cDNA synthesis, but poly(A) + mRNA may give higher yields and improved purity of final
Product Overview
- Simple, fast, and sensitive first-strand cDNA synthesis kit for RT-qPCR or RT-PCR
- Preblended 5X supermix contains all the necessary components except RNA template for reverse transcription
- Enhanced, engineered MMLV Rtase has reduced RNase H activity and contains a fidelity-enhancing subunit for superior accuracy
- Potent blend of RNase inhibitor prevents RNA degradation during reaction setup and reverse transcription
- Contains optimized blend of oligo(dT) and random primers for complete RNA coverage
- Complete cDNA synthesis and PCR the same day with 15 minute (for qPCR) or 50 minute (for PCR) cDNA synthesis protocol
- Both poly(A) + mRNA and total RNA can be used for first-strand cDNA synthesis, but poly(A) + mRNA may give higher yields and improved purity
Technical Documents
Technical Documents
Technical Documents
Detailed Description
Apex 5X all-in-one cDNA synthesis supermix is a convenient, ready-to-use formulation of all the reagents necessary for first-strand cDNA synthesis with the exception of the template. This optimized, 5X concentrated reaction master mix contains proprietary Reverse Transcriptase, Ribonuclease Inhibitor, dNTPs and a finely balanced ratio of Oligo(dT)s and random primers.
Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR/qPCR or placed in long-term storage at -20°C.
3 Product Options
Catalog Number:
42-702D
List Price:
$3734.8
Your Price:
$3201.3
Specifications:
| Size | 500 Reactions |
| Description | First-strand cDNA synthesis |
| Qty/Unit | 500 x 20ul Reactions/Unit |
Catalog Number:
42-702B
List Price:
$554.05
Your Price:
$474.9
Specifications:
| Size | 50 Reactions |
| Description | First-strand cDNA synthesis |
| Qty/Unit | 50 x 20ul Reactions/Unit |
Catalog Number:
42-702C
List Price:
$1037.45
Your Price:
$889.3
Specifications:
| Size | 100 Reactions |
| Description | First-strand cDNA synthesis |
| Qty/Unit | 100 x 20ul Reactions/Unit |
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