Cat #: 25-405
NEST 211262 NEST GelNest Matrix, High Concentration, 5 ml, Phenol Red-Free, LDEV-Free, 1 Bottle/Unit
Cat #: 25-405
NEST 211262 NEST GelNest Matrix, High Concentration, 5 ml, Phenol Red-Free, LDEV-Free, 1 Bottle/Unit
Phenol Red-Free, LDEV-Free
1 Bottle/Unit
Brand: NEST- Ideal for cell growth and differentiation assays
- Ideal for organoid cultures
- Metabolism/Toxicology Studies
- Use for angiogenesis and tumor invasion studies
- Guaranteed protein concentration of 20mg/mL
- Lot tested free of LDEV, bacteria and mycoplasma
- Lot tested endotoxin levels <10EU/mL
$192.45
Phenol Red-Free, LDEV-Free
1 Bottle/Unit
Brand: NEST- Ideal for cell growth and differentiation assays
- Ideal for organoid cultures
- Metabolism/Toxicology Studies
- Use for angiogenesis and tumor invasion studies
- Guaranteed protein concentration of 20mg/mL
- Lot tested free of LDEV, bacteria and mycoplasma
- Lot tested endotoxin levels <10EU/mL
GelNest is a basement membrane product identical in composition and performance to Corning® Matrigel® Matrix. GelNest Matrix extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcomas using genetically identical mouse lines and SOP's. EHS tumors contain plentiful ECM proteins such as laminin (60% ), collagen IV (30%), heparan sulfate proteoglycans, nidogen (8%), and several growth factors. Part number 211262 is a high protein concentration version of GelNest Matrix without phenol red indicator included. While normal GelNest Matrix has a protein concentration of >8mg/mL, high protein concentration GelNest Matrix features a concentration >18mg/mL. This formulation is also free of phenol red which can cause unwanted estrogenic effects in some cell lines. Being phenol red free also makes this formulation suitable for colorimetric assays. Equivalent to Corning: 354262. Ideal for :Cell Growth and Differentiation Assays, Metabolism/Toxicology Studies, Cell Invasion Assays, in vitro and in vivo Angiogenesis Studies, in vivo Angiogenesis Experiments and Augmentation of Tumors in Immunosuppressed Mice. Quality is guaranteed, each lot is produced and tested under stringent quality controls: Mouse colonies regularly tested for pathogens using mouse antibody production (MAP) protocol. Murine Engelbreth-Holm-Swarm (EHS) tumors used guaranteed free of LDEV. Per lot PCR testing for a wide variety of pathogens including LDEV. Tested and lot certified free of bacteria, fungi, and mycoplasma. Lowry method used to determine protein concentrations of >8mg/mL per lot. Limulus Amoebocyte Lysate assay used to quantify endotoxins, guaranteed <4EU/mL. Gel tested for 14 days at 37°C to guarantee stability. Lot tested using for biological activity using neurite growth and organoid formation assays.
211262 is a high protein concentration version of GelNest Matrix without phenol red indicator included. While normal GelNest Matrix has a protein concentration of >8mg/mL, high protein concentration GelNest Matrix features a concentration >18mg/mL. This formulation is also free of phenol red which can cause unwanted estrogenic effects in some cell lines. Being phenol red free also makes this formulation suitable for colorimetric assays.
Protein Concentration | 18-22mg |
---|---|
Storage Temp | -80°C |
Liquid Working Temperature | 4°C |
Gels At | 37°C |
Shelf Life | 7 years |
Volume | 5mL |
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