Cat #: 17-204B
PCR Biosystems PB10.23-10 PCRBIO Hot Start Taq Mix, Red, 25 x 1ml Hot Start Taq Mix Red, 1000 Reactions/Unit
Cat #: 17-204B
PCR Biosystems PB10.23-10 PCRBIO Hot Start Taq Mix, Red, 25 x 1ml Hot Start Taq Mix Red, 1000 Reactions/Unit
25 x 1ml Hot Start Taq Mix Red
1000 Reactions/Unit
Brand: PCR Biosystems- Proprietary hot start technology for unrivalled detection of low copy number templates
- Increased PCR success rates with amplicons up to 6kb
- Advanced buffer chemistry including Mg and dNTPs
- High yields under standard and fast PCR conditions
- Efficient specific amplification from complex templates including GC rich and AT rich sequences
- Load directly on agarose gels, contains red tracking dye
- Also available without dye and as a polymerase with separate reaction buffer
Color
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$1,043.20
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25 x 1ml Hot Start Taq Mix Red
1000 Reactions/Unit
Brand: PCR Biosystems- Proprietary hot start technology for unrivalled detection of low copy number templates
- Increased PCR success rates with amplicons up to 6kb
- Advanced buffer chemistry including Mg and dNTPs
- High yields under standard and fast PCR conditions
- Efficient specific amplification from complex templates including GC rich and AT rich sequences
- Load directly on agarose gels, contains red tracking dye
- Also available without dye and as a polymerase with separate reaction buffer
PCRBIO Hot Start Taq DNA Polymerase from Genesee Scientific uses advanced antibody-mediated hot start technology for superior sensitivity. Whether you need a hot start assay for high throughput, automated reaction set up or for detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.
“hot start” is a term used to describe the inactivation of a DNA polymerase until the initial activation step at 95°C. Inactivation below 65°C prevents primer-dimer formation and non-specific amplification allowing for specific amplification from low copy number target sequences. Our proprietary small-molecule hot start technology offers improved specificity and sensitivity compared to other methods. PCRBIO Hot Start DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO Hot Start Taq DNA Polymerase can perform consistently well on a broad range of templates (including both GC and AT rich).
| Packaging | 25 x 1ml HS Taq Master Mix Red |
|---|---|
| Reactions | 1000 x 50ul Reactions |
| Error Rate | 1 error per 2.0 x 10^5 nucleatides incorporated |
| Storage | Between -30 and -15°C |
| Color | Red |
| Compare To | DreamTaq™ Hot Start PCR Master Mix, Promega GoTaq® Hot Start Master Mixes |
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Can PCRBIO Hot Start Taq DNA Polymerase be used for Multiplex PCR?
Our PCRBIO Hot Start Taq DNA Polymerase is particularly suited for multiplexing due to the presence of our hot-start technology, which blocks the activity of Taq polymerase at low temperatures. When first performing multiplex PCR, we recommend running an annealing temperature gradient from 55°C to 65°C. The annealing temperature that results in the best specificity should be used in subsequent experiments. Fast cycling conditions should not be used for multiplex PCR. We recommend a 90 second extension time to begin with and this time may be extended to increase yield. For multiplex reactions, the reactions should be set up on ice or cooling blocs from start till finish. Primers must be designed carefully to avoid overlapping sequences as much as possible while maintaining diverse amplicon lengths that can be easily analyzed with your end-detection method
Yes. If you’re working from bacterial colonies use a sterile tip to pick a colony and re-suspend into the 50µl PCR reaction. If working from liquid culture add 5µl of overnight culture to the final mix. Follow the general protocol and increase the initial denaturation time to 10 min at 95°C.
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