Cat #: 17-104
PCR Biosystems PB10.41-10 PCRBIO HiFi Polymerase, 1 x 100ul Polymerase & 5 x 100ul Buffer, 200U/Unit
Cat #: 17-104
PCR Biosystems PB10.41-10 PCRBIO HiFi Polymerase, 1 x 100ul Polymerase & 5 x 100ul Buffer, 200U/Unit
1 x 100ul Polymerase & 5 x 100ul Buffer
200U/Unit
Brand: PCR Biosystems- 50x higher fidelity than Taq DNA polymerase
- Increased PCR success rates with amplicons up to 10kb
- Advanced buffer chemistry including Mg and dNTPs
- High yields under standard and fast PCR conditions
- Efficient specific amplification from complex templates including GC rich and AT rich sequences
Size
$193.20
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1 x 100ul Polymerase & 5 x 100ul Buffer
200U/Unit
Brand: PCR Biosystems- 50x higher fidelity than Taq DNA polymerase
- Increased PCR success rates with amplicons up to 10kb
- Advanced buffer chemistry including Mg and dNTPs
- High yields under standard and fast PCR conditions
- Efficient specific amplification from complex templates including GC rich and AT rich sequences
PCRBIO HiFi Polymerase from Genesee Scientific was derived from Pfu DNA polymerase for its’ 3’-5’ exonuclease (proofreading) activity in PCR. Several proprietary point-mutations allow for significantly improved performance when compared with its native form.
Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR. Enhanced DNA binding allows for improved processivity, increasing yield and shortening cycling times. The enhanced efficiency of PCRBio HiFi Polymerase minimizes PCR inhibition, from impure samples such as colony PCR and direct PCR. PCRBIO HiFi Polymerase uses the latest developments in DNA Polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO HiFi Polymerase can perform consistently well on a broad range of templates (including both GC and AT rich).
| Packaging | 1 x 100ul Polymerase & 5 x 100ul Buffer |
|---|---|
| Quantity | 200U/Unit |
| Error Rate | 1 error per 4.5 x 10^7 nucleatides incorporated |
| Storage | Between -30 and -15°C |
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What is the difference between PCRBIO HiFi Polymerase and PCRBIO VeriFi Polymerase?
PCRBIO VeriFi Polymerase has an enhanced processivity relative to PCRBIO HiFi Polymerase, resulting in shorter extension times (10-30s/kb), higher yields and the ability to amplify longer (up to 17.5kb) and more difficult targets. PCRBIO VeriFi Polymerase also has a higher fidelity than PCRBIO HiFi Polymerase. Unlike PCRBIO HiFi polymerase, PCRBIO VeriFi Polymerase is available as a convenient 2x ready mix with the option of a red dye for direct gel loading, saving time during reaction setup and analysis.
Yes. If you’re working from bacterial colonies use a sterile tip to pick a colony and re-suspend into the 50µl PCR reaction. If working from liquid culture add 5µl of overnight culture to the final mix. Follow the general protocol and increase the initial denaturation time to 10 min at 95°C.
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