Cat #: 13-041

Intact Genomics 1234-48 ig® 5-alpha DH5, Electrocompetent Cells, 12 x 100µl/Unit

Intact Genomics 1234-48 ig
Intact Genomics 1234-48 ig
Intact Genomics 1234-48 ig

Cat #: 13-041

Intact Genomics 1234-48 ig® 5-alpha DH5, Electrocompetent Cells, 12 x 100µl/Unit


Electrocompetent Cells

12 x 100µl/Unit

Brand: Intact Genomics

  • Suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning
  • Contains ?2 x 1010cfu/µg efficiency with electroporation
  • Greatly increase plasmid yield and quality due to endA1 mutation
  • High-efficiency transformation with plasmids 30 kb in size
  • Blue/white screening of recombinant clones due to lacZ?M15
  • Ensured insert stability due to recA1 mutation
  • Includes: ig™ 5-alpha electroCompetent cells, pUC19 control DNA, and recovery medium

Guest Price:
$390.00
List Price: $429.00
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Electrocompetent Cells

12 x 100µl/Unit

Brand: Intact Genomics

  • Suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning
  • Contains ?2 x 1010cfu/µg efficiency with electroporation
  • Greatly increase plasmid yield and quality due to endA1 mutation
  • High-efficiency transformation with plasmids 30 kb in size
  • Blue/white screening of recombinant clones due to lacZ?M15
  • Ensured insert stability due to recA1 mutation
  • Includes: ig™ 5-alpha electroCompetent cells, pUC19 control DNA, and recovery medium

Intact Genomics 5-alpha electrocompetent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning. Mutations in endA1 and recA1 ensure increased plasmid yield and improved plasmid quality.

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ?2 x 1010 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

Competent cell type Electrocompetent
Derivative of DH5-alpha
Species E. coli
Format Tubes
Transformation efficiency ?2 x 1010 cfu/µg pUC19 DNA
Blue/white screening Yes
Shipping condition Dry ice

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