Cat #: 11-736
Zymo Research D5465 Zymo-Seq WGBS Library Kit, NGS, 24 Preps/Unit
Cat #: 11-736
Zymo Research D5465 Zymo-Seq WGBS Library Kit, NGS, 24 Preps/Unit
NGS
24 Preps/Unit
Brand: Zymo Research- Whole Genome Bisulfite (WGBS) library preparation in less than 4 hours and in one tube
- Reduced library preparation bias for accurate methylation calling
- Reproducible genome coverage from any species (>90% of CpG sites detected in mammalian samples)
$1,082.45
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NGS
24 Preps/Unit
Brand: Zymo Research- Whole Genome Bisulfite (WGBS) library preparation in less than 4 hours and in one tube
- Reduced library preparation bias for accurate methylation calling
- Reproducible genome coverage from any species (>90% of CpG sites detected in mammalian samples)
Zymo-Seq WGBS Library Kit is the only kit available for whole genome bisulfite (WGBS) library preparation in a single tube. Zymo-Seq WGBS Library Kit incorporates tagmentation technology to eliminate the tedious fragmentation, enzymatic, and clean-up steps required by conventional, ligation-based library preparation methods. This streamlined workflow minimizes hands-on time, making it an ideal choice for higher throughput applications. To prepare Zymo-Seq WGBS libraries, intact genomic DNA is first bisulfite converted. Then, the following library preparation procedures are completed in a single tube: (1) second-strand synthesis, (2) adapterization via tagmentation, and (3) library amplification and indexing. After purification, libraries are ready for sequencing on Illumina instruments.
| DNA input | 10 - 100ng Ideally 100ng DNA in water, TE, or low salt buffer free of enzymatic inhibitors and RNA contamination. |
|---|---|
| Processing Time | ~4 hours |
| Required Material | Illumina DNA Prep Kit (Illumina, Cat. No. 20018704 or 20018705) |
| Sequencing Platform | all Illumina sequencing platforms (MiSeq, MiniSeq, HiSeq, NextSeq, NovaSeq) |
| Storage Condition | -80C |
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Does the Zymo-Seq WGBS Library Kit offer all the necessary reagents to generate libraries for sequencing?
No. The Illumina DNA Prep Kit (Cat. No. 20018704 or 20018705) is required and must be purchased directly from Illumina.
No. The kit provides twelve (12) Unique Dual Index Primer Sets.
The size of the input genomic DNA is important for high-quality and high-yield library. DNA from FFPE samples are often highly degraded, so the quality of the library will be affected. Cell-free DNA are not compatible with the kit because the fragment size is too short for efficient tagmentation.
Zymo-Seq WGBS Library Kit is compatible with genomic DNA extracted from any species, whether it is mammalian, bacteria, or plants. It is best to use high-quality, intact genomic DNA that is RNA-free.
Zymo-Seq WGBS kit is ideal for those working with higher inputs (10-100ng) of genomic DNA. It has minimal handling and clean-up steps, which makes it ideal for high-throughput library generation. Pico Methyl-Seq would be a better option for those working with small DNA inputs (< 10ng) or highly fragmented DNA, such as cfDNA or FFPE DNA.
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