Frequently Asked Questions
Does the Zymo-Seq WGBS Library Kit offer all the necessary reagents to generate libraries for sequencing?
No. The Illumina DNA Prep Kit (Cat. No. 20018704 or 20018705) is required and must be purchased directly from Illumina.
Do I need to purchase indexing primers separately?
No. The kit provides twelve (12) Unique Dual Index Primer Sets.
Can I use degraded samples, such as FFPE DNA or cell-free DNA to generate WGBS libraries from this kit?
The size of the input genomic DNA is important for high-quality and high-yield library. DNA from FFPE samples are often highly degraded, so the quality of the library will be affected. Cell-free DNA are not compatible with the kit because the fragment size is too short for efficient tagmentation.
What type of inputs is the Zymo-Seq WGBS Library Kit compatible with?
Zymo-Seq WGBS Library Kit is compatible with genomic DNA extracted from any species, whether it is mammalian, bacteria, or plants. It is best to use high-quality, intact genomic DNA that is RNA-free.